Applied Microbiology and Biotechnology

, Volume 89, Issue 5, pp 1387–1394

Production of serotonin by dual expression of tryptophan decarboxylase and tryptamine 5-hydroxylase in Escherichia coli

  • Sangkyu Park
  • Kiyoon Kang
  • Shin Woo Lee
  • Mi-Jeong Ahn
  • Jung-Myung Bae
  • Kyoungwhan Back
Biotechnological Products and Process Engineering

DOI: 10.1007/s00253-010-2994-4

Cite this article as:
Park, S., Kang, K., Lee, S.W. et al. Appl Microbiol Biotechnol (2011) 89: 1387. doi:10.1007/s00253-010-2994-4

Abstract

A plant-specific biogenic amine, serotonin, was produced by heterologous expression of two key biosynthetic genes, tryptophan decarboxylase (TDC) and tryptamine 5-hydroxylase (T5H), in Escherichia coli. The native T5H, a cytochrome P450 enzyme, was unable to be functionally expressed in E. coli. Through a series of N-terminal deletions or additions of tagging proteins, we generated a functional T5H enzyme construct (GST∆37T5H) in which glutathione S transferase (GST) was translationally fused with the N-terminal 37 amino acid deleted T5H. Dual expression of GST∆37T5H and TDC using a pCOLADuet-1 E. coli vector produced serotonin at concentrations of approximately 24 mg l−1 in the culture medium and 4 mg l−1 in the cells. An optimum temperature of approximately 20°C was required to achieve peak serotonin production in E. coli because the low induction temperature gave rise to the highest soluble expression of GST∆37T5H.

Keywords

Tryptophan decarboxylase Tryptamine 5-hydroxylase Dual expression Serotonin Escherichia coli 

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Sangkyu Park
    • 1
  • Kiyoon Kang
    • 1
  • Shin Woo Lee
    • 2
  • Mi-Jeong Ahn
    • 2
  • Jung-Myung Bae
    • 3
  • Kyoungwhan Back
    • 1
    • 4
  1. 1.Department of Biotechnology, Interdisciplinary Programs of Graduate School for Bioenergy and Biomaterials, Bioenergy Research CenterChonnam National UniversityGwangjuSouth Korea
  2. 2.Department of Crop Science & BiotechnologyJinju National UniversityJinjuSouth Korea
  3. 3.School of Life Sciences and BiotechnologyKorea UniversitySeoulSouth Korea
  4. 4.Department of Biotechnology, College of Agriculture and Life SciencesChonnam National UniversityGwangjuRepublic of Korea