Applied Microbiology and Biotechnology

, Volume 89, Issue 3, pp 835–842

Bacterial community analysis of swine manure treated with autothermal thermophilic aerobic digestion

  • Il Han
  • Shankar Congeevaram
  • Dong-Won Ki
  • Byoung-Taek Oh
  • Joonhong Park
Environmental Biotechnology

DOI: 10.1007/s00253-010-2893-8

Cite this article as:
Han, I., Congeevaram, S., Ki, DW. et al. Appl Microbiol Biotechnol (2011) 89: 835. doi:10.1007/s00253-010-2893-8

Abstract

Due to the enviornmental problems associated with disposal of livestock sludge, many stabilization studies emphasizing on the sludge volume reduction were performed. However, little is known about the microbial risk present in sludge and its stabilized products. This study microbiologically explored the effects of anaerobic lagoon fermentation (ALF) and autothermal thermophilic aerobic digestion (ATAD) on pathogen-related risk of raw swine manure by using culture-independent 16S rDNA cloning and sequencing methods. In raw swine manure, clones closely related to pathogens such as Dialister pneumosintes, Erysipelothrix rhusiopathiae, Succinivibrioan dextrinosolvens, and Schineria sp. were detected. Meanwhile, in the mesophilic ALF-treated swine manure, bacterial community clones closely related to pathogens such as Schineria sp. and Succinivibrio dextrinosolvens were still detected. Interestingly, the ATAD treatment resulted in no detection of clones closely related to pathogens in the stabilized thermophilic bacterial community, with the predominance of novel Clostridia class populations. These findings support the superiority of ATAD in selectively reducing potential human and animal pathogens compared to ALF, which is a typical manure stabilization method used in livestock farms.

Keywords

Livestock sludgeHuman and animal pathogensLivestock sludge stabilizationAutothermal thermophilic aerobic digestionMicrobial community analysis

Supplementary material

253_2010_2893_MOESM1_ESM.doc (32 kb)
Supplementary Table 1Average removals of organics, volatile suspended solids (VSS), and nutrients from swine manure after 4 days of the pilot-scale ATAD operation (unit = mg/L) (DOC 32 kb)
253_2010_2893_MOESM2_ESM.doc (151 kb)
Supplementary Table 2Clone sequence IDs and their GenBank accession numbers (DOC 151 kb)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Il Han
    • 1
  • Shankar Congeevaram
    • 1
    • 3
  • Dong-Won Ki
    • 1
  • Byoung-Taek Oh
    • 2
  • Joonhong Park
    • 1
    • 3
  1. 1.School of Civil and Environmental EngineeringYonsei UniversitySeoulSouth Korea
  2. 2.Division of Biotechnology, College of Environmental and Bioresource SciencesChonbuk National UniversityIksanSouth Korea
  3. 3.WCU Center for Green Metagenomics, Yonsei UniversitySeoulSouth Korea