Applied Microbiology and Biotechnology

, Volume 89, Issue 1, pp 121–130

Structural and functional analysis of hybrid enzymes generated by domain shuffling between Saccharomyces cerevisiae (var. diastaticus) Sta1 glucoamylase and Saccharomycopsis fibuligera Bgl1 β-glucosidase

Authors

  • Julia Marín-Navarro
    • Instituto de Agroquímica y Tecnología de AlimentosCSIC
  • Leontina Gurgu
    • Instituto de Agroquímica y Tecnología de AlimentosCSIC
    • Faculty of Food Science and EngineeringDunarea de Jos University
  • Santiago Alamar
    • Instituto de Agroquímica y Tecnología de AlimentosCSIC
    • Instituto de Agroquímica y Tecnología de AlimentosCSIC
    • Instituto de Agroquímica y Tecnología de AlimentosConsejo Superior de Investigaciones Científicas
Applied Genetics and Molecular Biotechnology

DOI: 10.1007/s00253-010-2845-3

Cite this article as:
Marín-Navarro, J., Gurgu, L., Alamar, S. et al. Appl Microbiol Biotechnol (2011) 89: 121. doi:10.1007/s00253-010-2845-3

Abstract

Saccharomyces cerevisiae Sta1 glucoamylase and Saccharomycopsis fibuligera Bgl1 β-glucosidase, two relevant enzymes from a biotechnological point of view, are proteins with multidomain structure. Starting with homology-based structural models of Sta1 and Bgl1, we have constructed a series of hybrid enzymes by interchanging domains of the two proteins. The first purpose of these constructs was to check available hypotheses about the uncertain biological functions of two domains: the serine/threonine-rich domain (STRD) of Sta1 and a β-sandwich domain present in Bgl1 that we have designated fibronectin-like domain (FLD). While, according to the initial hypothesis, proteins carrying the FLD tend to adhere to the cell wall, our results argued against the idea of an involvement of the STRD in protein secretion that stemmed from the presence of similar domains in different proteins secreted by yeast. The second objective of this work was to increase the enzymatic repertoire by generating enzymes with new structural and functional properties.

Keywords

CellobioseFibronectin-like domainGlycoside hydrolasesProtein engineeringStarch

Copyright information

© Springer-Verlag 2010