Applied Microbiology and Biotechnology

, Volume 86, Issue 5, pp 1493–1501

Display of Candida antarctica lipase B on Pichia pastoris and its application to flavor ester synthesis

  • Guo-Dong Su
  • Deng-Feng Huang
  • Shuang-Yan Han
  • Sui-Ping Zheng
  • Ying Lin
Applied Genetics and Molecular Biotechnology

DOI: 10.1007/s00253-009-2382-0

Cite this article as:
Su, G., Huang, D., Han, S. et al. Appl Microbiol Biotechnol (2010) 86: 1493. doi:10.1007/s00253-009-2382-0
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Abstract

Two alternative cell-surface display systems were developed in Pichia pastoris using the α-agglutinin and Flo1p (FS) anchor systems, respectively. Both the anchor cell wall proteins were obtained originally from Saccharomyces cerevisiae. Candida antarctica lipase B (CALB) was displayed functionally on the cell surface of P. pastoris using the anchor proteins α-agglutinin and FS. The activity of CALB displayed on P. pastoris was tenfold higher than that of S. cerevisiae. The hydrolytic and synthetic activities of CALB fused with α-agglutinin and FS anchored on P. pastoris were investigated. The hydrolytic activities of both lipases displayed on yeast cells surface were more than 200 U/g dry cell after 120 h of culture (200 and 270 U/g dry cell, respectively). However, the synthetic activity of CALB fused with α-agglutinin on P. pastoris was threefold higher than that of the FS fusion protein when applied to the synthesis of ethyl caproate. Similarly, the CALB displayed on P. pastoris using α-agglutinin had a higher catalytic efficiency with respect to the synthesis of other short-chain flavor esters than that displayed using the FS anchor. Interestingly, for some short-chain esters, the synthetic activity of displaying CALB fused with α-agglutinin on P. pastoris was even higher than that of the commercial CALB Novozyme 435.

Keywords

Yeast surface displayPichia pastorisCandida antarctica lipase BFlavor ester synthesis

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Guo-Dong Su
    • 1
  • Deng-Feng Huang
    • 1
  • Shuang-Yan Han
    • 1
  • Sui-Ping Zheng
    • 1
  • Ying Lin
    • 1
  1. 1.Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and BioengineeringSouth China University of TechnologyGuangzhouPeople’s Republic of China