Applied Microbiology and Biotechnology

, Volume 86, Issue 2, pp 731–741

Synthesis of γ-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463: functional grape must beverage and dermatological applications

Authors

  • Raffaella Di Cagno
    • Dipartimento di Protezione delle Piante e Microbiologia Applicata, Facoltà di AgrariaUniversità degli Studi di Bari
  • Francesco Mazzacane
    • Dipartimento di Protezione delle Piante e Microbiologia Applicata, Facoltà di AgrariaUniversità degli Studi di Bari
    • Dipartimento di Protezione delle Piante e Microbiologia Applicata, Facoltà di AgrariaUniversità degli Studi di Bari
  • Maria De Angelis
    • Dipartimento di Protezione delle Piante e Microbiologia Applicata, Facoltà di AgrariaUniversità degli Studi di Bari
  • Giammaria Giuliani
    • Giuliani S.p.A.
  • Marisa Meloni
    • In Vitro Research LaboratoriesVitroScreen Srl
  • Barbara De Servi
    • In Vitro Research LaboratoriesVitroScreen Srl
  • Marco Gobbetti
    • Dipartimento di Protezione delle Piante e Microbiologia Applicata, Facoltà di AgrariaUniversità degli Studi di Bari
Applied Microbial and Cell Physiology

DOI: 10.1007/s00253-009-2370-4

Cite this article as:
Di Cagno, R., Mazzacane, F., Rizzello, C.G. et al. Appl Microbiol Biotechnol (2010) 86: 731. doi:10.1007/s00253-009-2370-4

Abstract

Agriculture surplus were used as substrates to synthesize γ-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463 for the manufacture of a functional beverage or as a novel application for dermatological purposes. Dilution of the grape must to 1 or 4% (w/v) of total carbohydrates favored higher cell yield and synthesis of GABA with respect to whey milk. Optimal conditions for synthesizing GABA in grape must were: initial pH 6.0, initial cell density of Log 7.0 cfu/mL, and addition of 18.4 mM l-glutamate. L. plantarum DSM19463 synthesized 4.83 mM of GABA during fermentation at 30°C for 72 h. The fermented grape must also contain various levels of niacin, free minerals, and polyphenols, and Log 10.0 cfu/g of viable cells of L. plantarum DSM19463. Freeze dried preparation of grape must was applied to the SkinEthic® Reconstructed Human Epidermis or multi-layer human skin model (FT-skin tissue). The effect on transcriptional regulation of human beta-defensin-2 (HBD-2), hyaluronan synthase (HAS1), filaggrin (FGR), and involucrin genes was assayed through RT-PCR. Compared to GABA used as pure chemical compound, the up-regulation HBD-2 was similar while the effect on the expression of HAS1 and FGR genes was higher.

Keywords

γ-Aminobutyric acidFunctional grape mustHuman skin protectionLactobacillus plantarum

Copyright information

© Springer-Verlag 2009