Molecular design of yeast cell surface for adsorption and recovery of molybdenum, one of rare metals
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- Nishitani, T., Shimada, M., Kuroda, K. et al. Appl Microbiol Biotechnol (2010) 86: 641. doi:10.1007/s00253-009-2304-1
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In modern industrial society, molybdenum is one of the important metals for development of the industry of rare metals. It is important to recycle the rare metals from wastes because they are technically and economically difficult to be dug and be purified, and they exist in only a few regions in the world. In this study, ModE protein derived from Escherichia coli, which is a molybdate-dependent transcriptional regulator with the ability to bind molybdate as a form of soluble molybdenum, was displayed on the yeast cell surface by α-agglutinin-based cell surface display system for the adsorption and recovery of molybdate. Displayed ModE, confirmed by immunofluorescence labeling, caught molybdate more preferably at pH 3.0 than at basic pH. Yeast cells displaying C-terminal domain of ModE, which lacks N-terminal DNA binding domain, more effectively adsorbed molybdate than those displaying full-length ModE, suggesting that the deletion of the domain unrelated to metal binding enhanced the binding ability. Our results indicated that the adsorption system on cell surface of yeast cells displaying ModE is effective not only for adsorption of molybdate as a rare metal bioadsorbent but also for the easy recovery of molybdate located on the cell surface.
KeywordsCell surface engineeringRare metalMolybdenumModEBioadsorption
“Rare metals” are globally scarce metal elements; the digging and extraction of which from raw materials in the ground are difficult from the viewpoint of technical and economical aspects. Today, rare metals with some useful characteristics are indispensable for high-tech products, such as specific steel, lubricants, spark plugs, electronic materials, pigments, filaments of fluorescent light, and cellular phones, because they have important and prominent roles in realizing downsizing, resistance to heat, weight saving, high performance, and energy saving, although they are not directly used as raw materials. Rare metals are unevenly distributed in only a few regions, such as Africa, China, Russia, and South America. Although they are widely used as new materials of surrounding livingware and important for high-tech industry, the price of rare metals tends to fluctuate owing to their unstable supply. Especially, since autumn of 2004, the international price of these metals has greatly increased and we now face difficultly in securing rare metals (InfoMine Inc, http://www.infomine.com/). Therefore, their recycling is now necessary in modern industrial societies for the additional reasons that the metal resource itself is finite and may reach the limit of storage.
In living organisms, metals are necessary as essential elements. Among them, molybdenum is an essential trace element that is used to provide a redox active center in the molybdopterin cofactor of a number of enzymes (Rajagopalan 1996; Kisher et al. 1997). This transient metal is bioavailable in the form of water-soluble molybdate (MoO42-). In bacteria and archaea, molybdate is transported by an ABC-type transporter comprising three proteins, ModA (periplasmic binding protein), ModB (membrane protein), and ModC (ATPase; Self et al. 2001). The expression of the modABCD operon of Escherichia coli is controlled by ModE-Mo. In the absence of the high-affinity molybdate transporter, molybdate is also transported by another ABC transporter that transports sulfate/thiosulfate as well as by a nonspecific anion transporter. Thus, ModE is a bacterial transcriptional regulator (McNicholas et al. 1998; Kuper et al. 2003) that orchestrates many aspects of molybdenum metabolism by binding to specific DNA sequences in a molybdate-dependent fashion (Hollenstein et al. 2007). ModE, activated by binding molybdate, acts as both a positive and a negative transcriptional regulator of operons involved in various aspects of molybdenum metabolism. The ModE polypeptide can be subdivided into four structural domains from the N terminus to the C terminus, namely, the N-terminal domain involved in DNA binding, a linker region, and two so-called mop domains (Ulrike et al. 2000). The C-terminal domain of ModE (120–262 amino acid residues) is the molybdate-binding domain, which consists of two tandem repeats of mop domains. These β-rich mop domains found in a number of bacterial proteins have the function of binding molybdate at interdomain interfaces.
Yeast cell surface engineering (Ueda and Tanaka 2000a, b) is an effective strategy for adsorption and recovery of various metal ions on the cell surface. For example, as a tool for bioremediation, heavy metal ions such as cadmium ions are effectively adsorbed by the cell-surface-engineered yeast displaying yeast metallothionein, which is a cysteine-rich metal-binding protein (Kuroda and Ueda 2006). Furthermore, the enhanced bioadsorption of metal ions on the yeast cell surface has been established in recent years (Kuroda and Ueda 2003, 2006). These studies suggest that adsorption of metal ions by a protein displayed on the microbial cell surface has several benefits (Kuroda et al. 2001): high surface area-to-volume ratio for adsorption (Walker et al. 1989), ability to adsorb or recover metals by controlling pH and salt concentration. Adsorption on the cell surface is more advantageous than intracellular adsorption for the reason that cell-surface adsorption enables the recovery and recycling of the metal adsorbed on the cell surface without disintegration of cells, although intracellular accumulation needs extraction after disruption of cells. This system would enable us not only to remove toxic heavy metals but also to adsorb and recover useful metals such as rare metals from the environment.
In this study, we attempted to construct the cell-surface-engineered yeast with molybdate-binding ability as a recycle system for rare metals by displaying the molybdate-binding protein (ModE) on the yeast cell surface. The adsorption and recovery of MoO42- by ModE-displaying yeast were evaluated, and the usability of cell-surface adsorption in the collection of rare metals is discussed.
Materials and methods
Strains and media
E. coli DH5α [F−, ϕ80dlacZ∆M15, ∆ (lacZYA-argF)U169, hsdR17 (r−K, m+K), recA1, endA1, deoR, thi-1, supE44, gyrA96, relA1, λ−] was used as a host cell for recombinant DNA manipulation. Saccharomyces cerevisiae Δsed1 mutant strain derived from BY4741 (MATa, his3, leu2, met15, ura3) was used as the host for construction of the cell surface display of proteins (Kuroda et al. 2009). E. coli was grown in Luria–Bertani medium [1% (w/v) tryptone, 0.5% (w/v) yeast extract, 0.5% (w/v) sodium chloride] containing 100 μg ml−1 ampicillin. The yeast was precultivated aerobically in synthetic dextrose (SD) medium [0.67% (w/v) yeast nitrogen base without amino acids (Difco, Detroit, USA), 2% (w/v) glucose, 0.5% (w/v) casamino acids, 0.002% (w/v) l-histidine, 0.003% (w/v) l-leucine, and 0.003% (w/v) l-methionine] and then cultivated in the same medium.
Construction of plasmids for cell-surface display
Primers used in this study
Transformation of S. cerevisiae
The lithium acetate method (Ito et al. 1983) was used for transformation of S. cerevisiae cells. The transformants were isolated by incubation on a plate of selective SD medium [0.002% (w/v) l-histidine, 0.003% (w/v) l-leucine, and 0.003% (w/v) l-methionine] containing 0.5% (w/v) casamino acids at 30°C for 48 h.
To confirm the display of full-length ModE and C-terminal domain of ModE on the yeast cell surface, immunofluorescence labeling of cells was performed using the FLAG epitope tag as described below. Cells were fixed with 3.7% (v/v) formaldehyde/phosphate-buffered saline (PBS; pH 7.4) for 1.5 h and incubated in PBS (pH 7.4) containing 1% (w/v) bovine serum albumin for 30 min prior to immunostaining. Mouse monoclonal anti-FLAG M2 antibody (Sigma Chemical Co., St. Louis, MO, USA) was used as the primary antibody at a dilution rate of 1:300. A mixture of cells and the antibody was incubated using a rotator for 1.5 h at room temperature. The cells were then washed with PBS (pH 7.4). Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Molecular Probes Inc., OR, USA) diluted at 1:300 was then reacted with the cells using a rotator for 1.5 h at room temperature. After washing with PBS (pH 7.4), the cells were suspended in 30 μl of PBS (pH 7.4) and observed by microscopy. Fluorescence was detected using an inverted microscope IX71 (Olympus, Tokyo, Japan) through a U-MNIBA2 mirror unit with a BP470-490 excitation filter, DM505 dichroic mirror, and a BA510-550 emission filter (Olympus). Live images were obtained using Aqua-Cosmos 2.0 software (Hamamatsu Photonics, Shizuoka, Japan) controlling a digital charge-coupled device camera (C4742-95-12ER, Hamamatsu Photonics).
Measurement of fluorescence intensity
To quantify the amount of ModE and C-terminal domain of ModE displayed on the yeast cell surface, fluorescence intensity was evaluated. After immunofluorescence labeling, the cells were precipitated by centrifugation at 800 × g and the concentration of yeast was adjusted to an OD600 of 3.0 with PBS (pH 7.4). The fluorescence intensity was measured using Fluoroskan Ascent FL (Labsystems, Helsinki, Finland) in a tissue-culture plate (353072 Multiwell 96-well; Becton-Dickinson Labware, Franklin Lakes, NJ, USA). These values of fluorescence intensity correspond to the amount of ModE or C-terminal domain of ModE displayed on the yeast cell surface.
Bioadsorption and recovery of molybdate by cell-surface-engineered yeast cells
initial metal concentration
metal concentration of supernatant after bioadsorption experiment
metal concentration after recovery experiment
Construction of plasmids for cell-surface display of ModE and C-terminal domain of ModE
Cell-surface display of ModE and C-terminal domain of ModE
Bioadsorption and recovery of molybdate by cell-surface-engineered yeasts
Bioadsorption efficiency of cell-surface-engineered yeasts in 100 μM Na2MoO4
C-terminal domain display
Surface-engineered yeast cells can be repeatedly used as bioadsorbents because the cells themselves are not damaged by metal ions in the environment for the reason that adsorbed metal ions are easily released from the cell surface. This recycle is important for the sustainability of natural resources in modern societies. The metal-adsorption ability of surface-engineered yeasts depends on the feature of the displayed metal-binding proteins. We focused on ModE as a molybdate-binding protein and attempted to display ModE on the yeast cell surface by cell surface engineering.
As shown in Fig. 4b, ModE displayed on the cell surface was effective for adsorption of molybdate. Furthermore, the displayed ModE did not show specific binding to other metals except for tungstate, an analog of molybdate. The results suggested that C-terminal domain of ModE is more effective than full-length ModE for adsorption. From the measurement of fluorescence intensity, the amount of C-terminal domain of ModE displayed on the yeast cell surface was about four times that of full-length ModE. This result indicates that the transport and targeting of full-length ModE on the cell surface may become less efficient than those of C-terminal domain during the expression of fusion genes. Western blot analysis of the cell-surface protein showed that the intensity of a single band of displayed full-length ModE was lower than that of the C-terminal domain of ModE (data not shown). The full-length ModE includes a DNA-binding domain, a linker domain, and a molybdate-binding domain. C-terminal domain includes a part of the linker domain and a molybdate-binding domain. Both types have a stretched three-dimensional structure, so that the structure formation including folding does not seem to affect display efficiency. A difference in the amount of displayed proteins between full-length ModE and C-terminal domain of ModE due to the efficiency of synthesis might affect adsorption ability. Further, C-terminal domain of ModE has the higher adsorption ability than full-length ModE. In full-length ModE, the accessibility of the molybdate-binding domain might be affected by N-terminal DNA-binding domain, because N terminus of displayed proteins is directed toward the outside of cells in α-agglutinin-based yeast cell surface display system. However, the molybdate-binding domain is exposed directly to molybdate ions in C-terminal domain of ModE displaying because of having only molybdate-binding domain. Therefore, it may be easier for cell-surface-engineered yeast cells to bind to molybdate ions. In full-length ModE, the ability of adsorption at pH 13.0 becomes lower than that at pH 7.0 (Fig. 4b). However, in C-terminal domain of ModE, the dependence of adsorption ability on pH was not found. This may explain why the structural change of a DNA-binding domain in full-length ModE has an influence on the ability of adsorption.
For recovery of the adsorbed molybdate from yeast cells, the cells were washed with solutions of various pHs (Kuroda and Ueda 2003, 2006), detergents, and denaturing agents, but no enhanced recovery of molybdate was observed. The recovery experiment was next performed by treating the molybdate-bound yeast cells with papain dissolved in various buffers [water, PBS (pH 7.4), 50 mM PIPES buffer (pH 7.5), and 50 mM TAPS buffer (pH 9.0)] (Fig. 5). The recovery efficiency was highest in use of PBS (pH 7.4). For the reuse of cell-surface-engineered yeast cells harboring pULD1-ModE-Ct and readsorption of molybdate, yeast cells treated with papain were recultivated. Recultivated yeast cells grew as well as cells before papain treatment, and the readsorption efficiency was comparable to initial adsorption efficiency (data not shown). This finding indicated that cell-surface-engineered yeast cells are available for recultivation as a novel bioadsorbent (totally, so-called “recycling”).
In this study, our results suggested that cell-surface-engineered yeast cells displaying ModE are effective for molybdate adsorption, which is the oxyanion form in environmental water. Our adsorption technique is important in modern industrial societies because molybdenum is a rare metal utilized in various fields such as those requiring new materials required for high-tech devices, including electronic materials. The design of cell-surface adsorption by yeast cell surface engineering would be advantageous for adsorption and recovery of various rare metal ions because metal-binding proteins are effectively displayed on the cell surface and can contribute to the effective recycling of rare metal ions from metal-containing water in many industrial processes.
This study was supported by the Institute for Fermentation, Osaka.