Applied Microbiology and Biotechnology

, Volume 85, Issue 6, pp 1751–1758

Engineering Klebsiella oxytoca for efficient 2, 3-butanediol production through insertional inactivation of acetaldehyde dehydrogenase gene

  • Xiao-Jun Ji
  • He Huang
  • Jian-Guo Zhu
  • Lu-Jing Ren
  • Zhi-Kui Nie
  • Jun Du
  • Shuang Li
Biotechnological Products and Process Engineering

DOI: 10.1007/s00253-009-2222-2

Cite this article as:
Ji, XJ., Huang, H., Zhu, JG. et al. Appl Microbiol Biotechnol (2010) 85: 1751. doi:10.1007/s00253-009-2222-2

Abstract

Ethanol was a major byproduct of 2,3-butanediol (2,3-BD) fermentation by Klebsiella oxytoca ME-UD-3. In order to achieve a high efficiency of 2,3-BD production, K. oxytoca mutants deficient in ethanol formation were successfully constructed by replace the aldA gene coding for aldehyde dehydrogenase with a tetracycline resistance cassette. The results suggested that inactivation of aldA led to a significantly improved 2,3-BD production. The carbon flux to 2,3-BD was enhanced by eliminating the byproducing ethanol and at the same time reducing the accumulation of another byproduct acetoin. At last, by fed-batch culturing of the mutant, the final 2,3-BD titer up to 130 g/l with the productivity of 1.63 g/l.h and the 2,3-BD yield relative to glucose of 0.48 g/g was obtained.

Keywords

2,3-butanediolKlebsiella oxytocaAldehyde dehydrogenaseEthanolAcetoin

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Xiao-Jun Ji
    • 1
  • He Huang
    • 1
  • Jian-Guo Zhu
    • 1
  • Lu-Jing Ren
    • 1
  • Zhi-Kui Nie
    • 1
  • Jun Du
    • 1
  • Shuang Li
    • 1
  1. 1.State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical EngineeringNanjing University of TechnologyNanjingPeople’s Republic of China