Applied Microbiology and Biotechnology

, Volume 83, Issue 2, pp 199–208

Rapid sampling devices for metabolic engineering applications

Mini-Review

DOI: 10.1007/s00253-009-1976-x

Cite this article as:
Schädel, F. & Franco-Lara, E. Appl Microbiol Biotechnol (2009) 83: 199. doi:10.1007/s00253-009-1976-x

Abstract

A number of rapid sampling devices for metabolic engineering applications have been developed over the last years with the purpose of the estimation of in vivo metabolic concentrations and dynamics. This review outlines the designs and characteristics as well as the developments and changes in diverse approaches over the years. Primary performance parameters for these constructions are sampling time and rate and, for an accurate representation of the in vivo condition in cells, the reproducibility of results and easy handling throughout the sampling operation.

Keywords

Metabolic profiling Quenching Rapid sampling Sequential sampling Stimulus response experiments 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Institute of Biochemical EngineeringTechnische Universität BraunschweigBraunschweigGermany

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