Applied Microbiology and Biotechnology

, 83:233

Paclitaxel production using co-culture of Taxus suspension cells and paclitaxel-producing endophytic fungi in a co-bioreactor

Biotechnological Products and Process Engineering

DOI: 10.1007/s00253-009-1856-4

Cite this article as:
Li, YC., Tao, WY. & Cheng, L. Appl Microbiol Biotechnol (2009) 83: 233. doi:10.1007/s00253-009-1856-4


The co-culture of the suspension cells of Taxus chinensis var. mairei and its endophytic fungi, Fusarium mairei, in a 20-L co-bioreactor was successfully established for paclitaxel production. The co-bioreactor consists of two-unit tanks (10 L each) with a repairable separate membrane in the center, culturing Taxus suspension cells in one tank and growing fungi in another. By optimizing the co-culture conditions, there was a desirable yield of paclitaxel in Taxus cell cultures. The Taxus cell cultures by co-culture produced 25.63 mg/L of paclitaxel within 15 days; it was equivalent to a productivity of 1.71 mg/L per day and 38-fold higher than that by uncoupled culture (0.68 mg/L within 15 days). The optimum conditions for co-culture in the co-bioreactor were: B5 medium, inoculating fungi when Taxus cells had grown for 5 days in the co-bioreactor, hydrophilic separate membrane in the center of the co-bioreactor, and air flow rate of 1:0.85 v/v/m in fungus cultures.


Biochemical engineeringCo-bioreactorCo-cultureEndophytic fungiPaclitaxelPlant cell suspension culture

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiPeople’s Republic of China
  2. 2.School of FoodHainan UniversityHaikouPeople’s Republic of China
  3. 3.Wuxi Higher Health Vocational Technology SchoolWuxiPeople’s Republic of China