Applied Microbiology and Biotechnology

, Volume 83, Issue 2, pp 217–223

A flow injection analysis system with encapsulated high-density Saccharomyces cerevisiae cells for rapid determination of biochemical oxygen demand

  • Kyo Seong Seo
  • Kwang Ho Choo
  • Ho Nam Chang
  • Joong Kon Park
Biotechnological Products and Process Engineering

DOI: 10.1007/s00253-008-1852-0

Cite this article as:
Seo, K.S., Choo, K.H., Chang, H.N. et al. Appl Microbiol Biotechnol (2009) 83: 217. doi:10.1007/s00253-008-1852-0

Abstract

The biochemical oxygen demand (BOD) determination was studied using a novel flow injection analysis (FIA) system with encapsulated Saccharomyces cerevisiae cells and an oxygen electrode and was compared with conventional 5-day BOD tests. S. cerevisiae cells were packed in a calcium alginate capsule at a dry cell weight of 250 g/l of capsule core. The level of dissolved oxygen (DO) was reduced due to the enhanced respiratory activity of the microbial cells when the injected nutrient passed through the bioreactor. The decrease in DO (ΔDO) was intensified with the amount of microbial cells packed in the bioreactor. However, the specific ΔDO decreased as the amount of cells loaded in the bioreactor increased. The ΔDO value was dependent on the pH and temperature of the mobile phase and reached its maximum value at 35°C and pH 7–8. Also, ΔDO became larger at longer response times as the flow rate of the mobile phase decreased. The measurement of ΔDO was repeated more than six times consecutively using a 20-ppm standard glucose and glutamic acid solution, which confirmed the reproducibility with a standard deviation of 0.95%. A strong linear correlation between ΔDO and BOD was also observed. The 5-day BOD values of actual water and wastewater samples were in accordance with the BOD values obtained by this FIA method using encapsulated S. cerevisiae cells. Unlike the cell-immobilized bead system, there was no contamination of the bioreactor resulting from any leak of yeast cells from the sensor capsules during BOD measurements.

Keywords

BOD sensor FIA Biochemical oxygen demand Encapsulation High cell density Saccharomyces cerevisiae 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Kyo Seong Seo
    • 1
  • Kwang Ho Choo
    • 2
  • Ho Nam Chang
    • 3
  • Joong Kon Park
    • 1
  1. 1.Department of Chemical EngineeringKyungpook National UniversityDaeguRepublic of Korea
  2. 2.Department of Environmental EngineeringKyungpook National UniversityDaeguRepublic of Korea
  3. 3.Department of Chemical and Biomolecular EngineeringKorea Advanced Institute of Science and TechnologyDaejeonRepublic of Korea

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