Applied Microbiology and Biotechnology

, Volume 82, Issue 1, pp 105–113

Creation of a novel peptide endowing yeasts with acid tolerance using yeast cell-surface engineering

Authors

  • Ken Matsui
    • Division of Applied Life Sciences, Graduate School of AgricultureKyoto University
  • Kouichi Kuroda
    • Division of Applied Life Sciences, Graduate School of AgricultureKyoto University
    • Division of Applied Life Sciences, Graduate School of AgricultureKyoto University
Applied Genetics and Molecular Biotechnology

DOI: 10.1007/s00253-008-1761-2

Cite this article as:
Matsui, K., Kuroda, K. & Ueda, M. Appl Microbiol Biotechnol (2009) 82: 105. doi:10.1007/s00253-008-1761-2

Abstract

The cell wall of Saccharomyces cerevisiae plays an essential role in the biophysical characteristics of the cell surface. The modification of the cell wall property is an important factor for cellular adaptation to a stressful environment. In this study, we randomly modified the cell wall by displaying combinatorial random peptides on the yeast cell surface, and by screening, we successfully obtained a novel peptide, Scr35, that endowed yeasts with acid tolerance. The yeast, surface-modified by Scr35, was able to grow well under acidic condition and low glucose condition and showed high glucose uptake activity. However, the growth of the modified yeast became inferior as extracellular pH became higher. This inferiority was rescued by decreasing glucose concentration in a medium. Our results suggest that the optimum pH of a medium becomes low when the newly created Scr35 affects glucose uptake activity through cell-surface modification. Therefore, such artificial modification of the cell surface has a great potential as a useful tool for breeding acid-tolerant yeasts for industrial applications of S. cerevisiae as a biocatalyst.

Keywords

YeastAcid tolerancePeptideCell-surface engineeringGlucose

Copyright information

© Springer-Verlag 2008