Applied Microbiology and Biotechnology

, Volume 81, Issue 5, pp 951–960

Fermentative capacity of dry active wine yeast requires a specific oxidative stress response during industrial biomass growth

  • Roberto Pérez-Torrado
  • Rocío Gómez-Pastor
  • Christer Larsson
  • Emilia Matallana
Applied Microbial and Cell Physiology

DOI: 10.1007/s00253-008-1722-9

Cite this article as:
Pérez-Torrado, R., Gómez-Pastor, R., Larsson, C. et al. Appl Microbiol Biotechnol (2009) 81: 951. doi:10.1007/s00253-008-1722-9

Abstract

Induction of the oxidative stress response has been described under many physiological conditions in Saccharomyces cerevisiae, including industrial fermentation for wine yeast biomass production where cells are grown through several batch and fed-batch cultures on molasses. Here, we investigate the influence of aeration on the expression changes of different gene markers for oxidative stress and compare the induction profiles to the accumulation of several intracellular metabolites in order to correlate the molecular response to physiological and metabolic changes. We also demonstrate that this specific oxidative response is relevant for wine yeast performance by construction of a genetically engineered wine yeast strain overexpressing the TRX2 gene that codifies a thioredoxin, one of the most important cellular defenses against oxidative damage. This modified strain displays an improved fermentative capacity and lower levels of oxidative cellular damages than its parental strain after dry biomass production.

Keyword

Wine yeast propagationOxidative stress responseFermentative capacityGenetic improvement

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Roberto Pérez-Torrado
    • 2
  • Rocío Gómez-Pastor
    • 2
  • Christer Larsson
    • 3
  • Emilia Matallana
    • 1
    • 2
  1. 1.Departamento de Bioquímica y Biología MolecularUniversitat de ValènciaValenciaSpain
  2. 2.Departamento de BiotecnologíaInstituto de Agroquímica y Tecnología de Alimentos, CSICValenciaSpain
  3. 3.Molecular Biotechnology, Department of Chemical and Biological EngineeringChalmers University of TechnologyGöteborgSweden