Applied Microbiology and Biotechnology

, Volume 79, Issue 2, pp 263–272

Development of chemically defined medium for Mannheimia succiniciproducens based on its genome sequence

  • Hyohak Song
  • Tae Yong Kim
  • Bo-Kyeong Choi
  • Seong Jun Choi
  • Lars K. Nielsen
  • Ho Nam Chang
  • Sang Yup Lee
Applied Microbial and Cell Physiology

DOI: 10.1007/s00253-008-1425-2

Cite this article as:
Song, H., Kim, T.Y., Choi, BK. et al. Appl Microbiol Biotechnol (2008) 79: 263. doi:10.1007/s00253-008-1425-2

Abstract

This study presents a novel methodology for the development of a chemically defined medium (CDM) using genome-scale metabolic network and flux balance analysis. The genome-based in silico analysis identified two amino acids and four vitamins as non-substitutable essential compounds to be supplemented to a minimal medium for the sustainable growth of Mannheimia succiniciproducens, while no substitutable essential compounds were identified. The in silico predictions were verified by cultivating the cells on a CDM containing the six non-substitutable essential compounds, and it was further demonstrated by observing no cell growth on the CDM lacking any one of the non-substitutable essentials. An optimal CDM for the enhancement of cell growth and succinic acid production, as a target product, was formulated with a single-addition technique. The fermentation on the optimal CDM increased the succinic acid productivity by 36%, the final succinic acid concentration by 17%, and the succinic acid yield on glucose by 15% compared to the cultivation using a complex medium. The optimal CDM also lowered the sum of the amounts of by-products (acetic, formic, and lactic acids) by 30%. The strategy reported in this paper should be generally applicable to the development of CDMs for other organisms, whose genome sequences are available.

Keywords

Chemically defined medium Mannheimia succiniciproducens Metabolic network Flux balance analysis Succinic acid 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Hyohak Song
    • 1
    • 2
    • 3
  • Tae Yong Kim
    • 1
    • 2
    • 3
  • Bo-Kyeong Choi
    • 1
    • 2
  • Seong Jun Choi
    • 1
    • 2
    • 3
  • Lars K. Nielsen
    • 5
  • Ho Nam Chang
    • 1
    • 2
  • Sang Yup Lee
    • 1
    • 2
    • 3
    • 4
  1. 1.Department of Chemical and Biomolecular Engineering (BK21 Program)KAISTDaejeonRepublic of Korea
  2. 2.BioProcess Engineering Research CenterKAISTDaejeonRepublic of Korea
  3. 3.Metabolic and Biomolecular Engineering National Research Laboratory, Center for Systems and Synthetic Biotechnology, Institute for the BioCenturyKAISTDaejeonRepublic of Korea
  4. 4.Department of Bio and Brain Engineering and Bioinformatics Research CenterKAISTDaejeonRepublic of Korea
  5. 5.Australian Institute for Bioengineering and NanotechnologyUniversity of QueenslandBrisbaneAustralia

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