Applied Microbiology and Biotechnology

, Volume 78, Issue 6, pp 939–945

NADH- vs NADPH-coupled reduction of 5-hydroxymethyl furfural (HMF) and its implications on product distribution in Saccharomyces cerevisiae


  • João R. M. Almeida
    • Department of Applied MicrobiologyLund University
  • Anja Röder
    • Department of Applied MicrobiologyLund University
  • Tobias Modig
    • Department of Chemical EngineeringLund University
  • Boaz Laadan
    • Department of Applied MicrobiologyLund University
  • Gunnar Lidén
    • Department of Chemical EngineeringLund University
    • Department of Applied MicrobiologyLund University
Biotechnological Products and Process Engineering

DOI: 10.1007/s00253-008-1364-y

Cite this article as:
Almeida, J.R.M., Röder, A., Modig, T. et al. Appl Microbiol Biotechnol (2008) 78: 939. doi:10.1007/s00253-008-1364-y


Saccharomyces cerevisiae alcohol dehydrogenases responsible for NADH-, and NADPH-specific reduction of the furaldehydes 5-hydroxymethyl-furfural (HMF) and furfural have previously been identified. In the present study, strains overexpressing the corresponding genes (mut-ADH1 and ADH6), together with a control strain, were compared in defined medium for anaerobic fermentation of glucose in the presence and absence of HMF. All strains showed a similar fermentation pattern in the absence of HMF. In the presence of HMF, the strain overexpressing ADH6 showed the highest HMF reduction rate and the highest specific ethanol productivity, followed by the strain overexpressing mut-ADH1. This correlated with in vitro HMF reduction capacity observed in the ADH6 overexpressing strain. Acetate and glycerol yields per biomass increased considerably in the ADH6 strain. In the other two strains, only the overall acetate yield per biomass was affected. When compared in batch fermentation of spruce hydrolysate, strains overexpressing ADH6 and mut-ADH1 had five times higher HMF uptake rate than the control strain and improved specific ethanol productivity. Overall, our results demonstrate that (1) the cofactor usage in the HMF reduction affects the product distribution, and (2) increased HMF reduction activity results in increased specific ethanol productivity in defined mineral medium and in spruce hydrolysate.


ADH1ADH6Hydroxymethyl furfuralSaccharomyces cerevisiaeLignocellulosic hydrolysate

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© Springer-Verlag 2008