Applied Microbiology and Biotechnology

, 78:643

Hide depilation and feather disintegration studies with keratinolytic serine protease from a novel Bacillus subtilis isolate

Biotechnologically Relevant Enzymes and Proteins

DOI: 10.1007/s00253-008-1355-z

Cite this article as:
Pillai, P. & Archana, G. Appl Microbiol Biotechnol (2008) 78: 643. doi:10.1007/s00253-008-1355-z

Abstract

Keratinases play an important role in biotechnological applications such as improvement of feather meal, enzymatic dehairing and production of amino acids or peptides from high molecular weight substrates. Bacillus subtilis P13, isolated from Vajreshwari hot spring (45–50°C) near Mumbai, India, produces a neutral serine protease and has an optimum temperature of 65°C. This enzyme preparation was keratinolytic in nature and could disintegrate whole chicken feathers, except for the remnants of shafts. The enzyme preparation also exhibited depilation of goat hides with the recovery of intact animal hair. The enzyme preparation could release peptides from ground feathers and bring about their weight reduction; however, similar action on hair was relatively weak. A single major PMSF-sensitive protease band could be detected upon zymogram analysis, indicating that a single enzyme may be responsible for feather degradation and hide depilation. The importance of these findings in the biotechnological application for feather and leather industries is discussed.

Keywords

KeratinaseBacillus subtilis P13Feather disintegrationHide dehairing

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Department of Microbiology and Biotechnology Centre, Faculty of ScienceThe Maharaja Sayajirao University of BarodaVadodaraIndia