Applied Microbiology and Biotechnology

, Volume 78, Issue 1, pp 29–36

High-density fermentation of microalga Chlorella protothecoides in bioreactor for microbio-diesel production

Biotechnological Products and Process Engineering

DOI: 10.1007/s00253-007-1285-1

Cite this article as:
Xiong, W., Li, X., Xiang, J. et al. Appl Microbiol Biotechnol (2008) 78: 29. doi:10.1007/s00253-007-1285-1


Agal-fermentation-based microbio-diesel production was realized through high-cell-density fermentation of Chlorella protothecoides and efficient transesterification process. Cell density achieved was 16.8 g l−1 in 184 h and 51.2 g l−1 in 167 h in a 5-l bioreactor by performing preliminary and improved fed-batch culture strategy, respectively. The lipid content was 57.8, 55.2, and 50.3% of cell dry weight from batch, primary, and improved fed-batch culture in 5-l bioreactor. Transesterification was catalyzed by immobilized lipase, and the conversion rate reached up to 98%. The properties of biodiesel from Chlorella were comparable to conventional diesel fuel and comply with US standard for Biodiesel. In a word, the approach including high-density fermentation of Chlorella and enzymatic transesterification process were set up and proved to be a promising alternative for biodiesel production.


Agal-fermentation-based microbio-diesel (AFMD)Chlorella protothecoidesFed-batchHigh-density fermentationImmobilized lipase

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Department of Biological Sciences and BiotechnologyTsinghua UniversityBeijingPeople’s Republic of China