Applied Microbiology and Biotechnology

, Volume 78, Issue 1, pp 157–163

Substrate specificity of Stenotrophomonas nitritireducens in the hydroxylation of unsaturated fatty acid

Authors

  • In-Sik Yu
    • Department of Bioscience and BiotechnologyKonkuk University
  • Soo-Jin Yeom
    • Department of Bioscience and BiotechnologyKonkuk University
  • Hye-Jung Kim
    • Department of Bioscience and BiotechnologyKonkuk University
  • Jung-Kul Lee
    • Department of Chemical EngineeringKonkuk University
  • Yong-Hwi Kim
    • Department of Food Science and TechnologySejong University
    • Department of Bioscience and BiotechnologyKonkuk University
Applied Microbial and Cell Physiology

DOI: 10.1007/s00253-007-1280-6

Cite this article as:
Yu, I., Yeom, S., Kim, H. et al. Appl Microbiol Biotechnol (2008) 78: 157. doi:10.1007/s00253-007-1280-6

Abstract

An isolated bacterium that converted unsaturated fatty acids to hydroxy fatty acids was identified as Stenotrophomonas nitritireducens by API analysis, cellular fatty acids compositions, sequencing the full 16S ribosomal ribonucleic acid, and evaluating its nitrite reduction ability. S. nitritireducens has unique regio-specificity for C16 and C18 cis-9 unsaturated fatty acids. These fatty acids are converted to their 10-hydroxy fatty acids without detectable byproducts. Among the cis-9-unsaturated fatty acids, S. nitritireducens showed the highest specificity for linoleic acid. The cells converted 20 mM linoleic acid to 13.5 mM 10-hydroxy-12(Z)-octadecenoic acid at 30°C and pH 7.5 with a yield of 67.5% (mol/mol).

Keywords

10-Hydroxy fatty acidsSubstrate specificityStenotrophomonas nitritireducenscis-9-Unsaturated fatty acids

Copyright information

© Springer-Verlag 2007