Applied Microbiology and Biotechnology

, Volume 77, Issue 6, pp 1219–1224

Production of isopropanol by metabolically engineered Escherichia coli


  • Toru Jojima
    • Research Institute of Innovative Technology for the Earth (RITE)
  • Masayuki Inui
    • Research Institute of Innovative Technology for the Earth (RITE)
    • Research Institute of Innovative Technology for the Earth (RITE)
Biotechnological Products and Process Engineering

DOI: 10.1007/s00253-007-1246-8

Cite this article as:
Jojima, T., Inui, M. & Yukawa, H. Appl Microbiol Biotechnol (2008) 77: 1219. doi:10.1007/s00253-007-1246-8


A genetically engineered strain of Escherichia coli JM109 harboring the isopropanol-producing pathway consisting of five genes encoding four enzymes, thiolase, coenzyme A (CoA) transferase, acetoacetate decarboxylase from Clostridium acetobutylicum ATCC 824, and primary–secondary alcohol dehydrogenase from C. beijerinckii NRRL B593, produced up to 227 mM of isopropanol from glucose under aerobic fed-batch culture conditions. Acetate production by the engineered strain was approximately one sixth that produced by a control E. coli strain bearing an expression vector without the clostridial genes. These results demonstrate a functional isopropanol-producing pathway in E. coli and consequently carbon flux from acetyl-CoA directed to isopropanol instead of acetate. This is the first report on isopropanol production by genetically engineered microorganism under aerobic culture conditions.


Isopropanol Escherichia coliClostridium acetobutylicumClostridium beijerinckiiMetabolic engineering

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© Springer-Verlag 2007