Applied Microbiology and Biotechnology

, Volume 77, Issue 6, pp 1219–1224

Production of isopropanol by metabolically engineered Escherichia coli

Authors

  • Toru Jojima
    • Research Institute of Innovative Technology for the Earth (RITE)
  • Masayuki Inui
    • Research Institute of Innovative Technology for the Earth (RITE)
    • Research Institute of Innovative Technology for the Earth (RITE)
Biotechnological Products and Process Engineering

DOI: 10.1007/s00253-007-1246-8

Cite this article as:
Jojima, T., Inui, M. & Yukawa, H. Appl Microbiol Biotechnol (2008) 77: 1219. doi:10.1007/s00253-007-1246-8

Abstract

A genetically engineered strain of Escherichia coli JM109 harboring the isopropanol-producing pathway consisting of five genes encoding four enzymes, thiolase, coenzyme A (CoA) transferase, acetoacetate decarboxylase from Clostridium acetobutylicum ATCC 824, and primary–secondary alcohol dehydrogenase from C. beijerinckii NRRL B593, produced up to 227 mM of isopropanol from glucose under aerobic fed-batch culture conditions. Acetate production by the engineered strain was approximately one sixth that produced by a control E. coli strain bearing an expression vector without the clostridial genes. These results demonstrate a functional isopropanol-producing pathway in E. coli and consequently carbon flux from acetyl-CoA directed to isopropanol instead of acetate. This is the first report on isopropanol production by genetically engineered microorganism under aerobic culture conditions.

Keywords

Isopropanol Escherichia coliClostridium acetobutylicumClostridium beijerinckiiMetabolic engineering

Copyright information

© Springer-Verlag 2007