Applied Microbiology and Biotechnology

, Volume 77, Issue 4, pp 909–915

Improved adaptation to heat, cold, and solvent tolerance in Lactobacillus plantarum

  • D. Fiocco
  • V. Capozzi
  • P. Goffin
  • P. Hols
  • Giuseppe Spano
Applied Genetics and Molecular Biotechnology

DOI: 10.1007/s00253-007-1228-x

Cite this article as:
Fiocco, D., Capozzi, V., Goffin, P. et al. Appl Microbiol Biotechnol (2007) 77: 909. doi:10.1007/s00253-007-1228-x

Abstract

The effect of overproducing each of the three small heat shock proteins (Hsp; Hsp 18.5, Hsp 18.55, and Hsp 19.3) was investigated in Lactobacillus plantarum strain WCFS1. Overproduction of the three genes, hsp 18.5, hsp 18.55, and hsp 19.3, translationally fused to the start codon of the ldhL gene yielded a protein of approximately 19 kDa, as estimated from Tricine sodium dodecyl sulfate–polyacrylamide gel electrophoresis in agreement with the predicted molecular weight of small Hsps. Small Hsp overproduction alleviated the reduction in growth rate triggered by exposing exponentially growing cells to heat shock (37 or 40°C) and cold shock (12°C). Moreover, overproduction of Hsp 18.55 and Hsp 19.3 led to an enhanced survival in the presence of butanol (1% v/v) or ethanol (12% v/v) treatment suggesting a potential role of L. plantarum small Hsps in solvent tolerance.

Keywords

Lactobacillus plantarumHeat shockOverexpression plasmidsSolvent tolerance

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • D. Fiocco
    • 1
    • 2
  • V. Capozzi
    • 1
    • 2
  • P. Goffin
    • 3
    • 4
  • P. Hols
    • 3
  • Giuseppe Spano
    • 1
    • 2
  1. 1.Department of Food ScienceFoggia UniversityFoggiaItaly
  2. 2.Bioagromed Centro di Ricerca InterdipartimentaleFoggiaItaly
  3. 3.Unité de Génétique, Institut des Sciences de la Vie (ISV)Université Catholique de LouvainLouvain-la-NeuveBelgium
  4. 4.NIZO food researchEdeThe Netherlands