Applied Microbiology and Biotechnology

, Volume 77, Issue 2, pp 339–346

Hyaluronic acid production by recombinant Lactococcus lactis

Authors

  • Liang-Jung Chien
    • Department of Chemical EngineeringNational Taiwan University of Science and Technology
    • Department of Chemical EngineeringNational Taiwan University of Science and Technology
Applied Genetics and Molecular Biotechnology

DOI: 10.1007/s00253-007-1153-z

Cite this article as:
Chien, L. & Lee, C. Appl Microbiol Biotechnol (2007) 77: 339. doi:10.1007/s00253-007-1153-z

Abstract

Microbial hyaluronic acid (HA), commonly produced by pathogenic Streptococcus, was made possible to be produced by a generally recognized as safe Lactococcus lactis by coexpressing HA synthase and uridine diphosphate–glucose dehydrogenase (UDP-GlcDH) of Streptococcus equi subsp. zooepidemicus in a nisin-controlled expression (NICE) system. With scarce expressed HA synthase alone, the constructed recombinant L. lactis (LL-NA) strain could produce HA with a concentration about 0.08 g/l in the M17 medium supplemented with 1% (w/v) glucose. In contrast to HA synthase, UDP-GlcDH of Streptococcus could be overexpressed in the NICE system. With coexpression of heterologous UDP-GlcDH with HA synthase, the constructed LL-NAB strain grew slightly slower to a concentration about 10% lower that of the LL-NA strain. However, the HA concentration produced was enhanced about eightfold to 0.65 g/l.

Keywords

Hyaluronic acidNisinNICELactococcus lactisHA synthase expression

Copyright information

© Springer-Verlag 2007