Applied Microbiology and Biotechnology

, Volume 77, Issue 2, pp 339–346

Hyaluronic acid production by recombinant Lactococcus lactis

Applied Genetics and Molecular Biotechnology

DOI: 10.1007/s00253-007-1153-z

Cite this article as:
Chien, LJ. & Lee, CK. Appl Microbiol Biotechnol (2007) 77: 339. doi:10.1007/s00253-007-1153-z


Microbial hyaluronic acid (HA), commonly produced by pathogenic Streptococcus, was made possible to be produced by a generally recognized as safe Lactococcus lactis by coexpressing HA synthase and uridine diphosphate–glucose dehydrogenase (UDP-GlcDH) of Streptococcus equi subsp. zooepidemicus in a nisin-controlled expression (NICE) system. With scarce expressed HA synthase alone, the constructed recombinant L. lactis (LL-NA) strain could produce HA with a concentration about 0.08 g/l in the M17 medium supplemented with 1% (w/v) glucose. In contrast to HA synthase, UDP-GlcDH of Streptococcus could be overexpressed in the NICE system. With coexpression of heterologous UDP-GlcDH with HA synthase, the constructed LL-NAB strain grew slightly slower to a concentration about 10% lower that of the LL-NA strain. However, the HA concentration produced was enhanced about eightfold to 0.65 g/l.


Hyaluronic acidNisinNICELactococcus lactisHA synthase expression

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Department of Chemical EngineeringNational Taiwan University of Science and TechnologyTaipeiTaiwan