Applied Microbiology and Biotechnology

, Volume 77, Issue 1, pp 167–173

Polyphosphate kinase genes from full-scale activated sludge plants

Authors

    • Department of Civil and Environmental EngineeringUniversity of Wisconsin–Madison
  • Suzan Yilmaz
    • Department of Civil and Environmental EngineeringUniversity of California at Berkeley
  • Shaomei He
    • Department of Civil and Environmental EngineeringUniversity of Wisconsin–Madison
  • Daniel L. Gall
    • Department of Civil and Environmental EngineeringUniversity of Wisconsin–Madison
  • David Jenkins
    • Department of Civil and Environmental EngineeringUniversity of California at Berkeley
  • Jay D. Keasling
    • Department of Chemical EngineeringUniversity of California at Berkeley
    • Department of BioengineeringUniversity of California at Berkeley
    • Physical Biosciences DivisionLawrence Berkeley National Laboratory
Environmental Biotechnology

DOI: 10.1007/s00253-007-1122-6

Cite this article as:
McMahon, K.D., Yilmaz, S., He, S. et al. Appl Microbiol Biotechnol (2007) 77: 167. doi:10.1007/s00253-007-1122-6

Abstract

The performance of enhanced biological phosphorus removal (EBPR) wastewater treatment processes depends on the presence of bacteria that accumulate large quantities of polyphosphate. One such group of bacteria has been identified and named Candidatus Accumulibacter phosphatis. Accumulibacter-like bacteria are abundant in many EBPR plants, but not much is known about their community or population ecology. In this study, we used the polyphosphate kinase gene (ppk1) as a high-resolution genetic marker to study population structure in activated sludge. Ppk1 genes were amplified from samples collected from full-scale wastewater treatment plants of different configurations. Clone libraries were constructed using primers targeting highly conserved regions of ppk1, to retrieve these genes from activated sludge plants that did, and did not, perform EBPR. Comparative sequence analysis revealed that ppk1 fragments were retrieved from organisms affiliated with the Accumulibacter cluster from EBPR plants but not from a plant that did not perform EBPR. A new set of more specific primers was designed and validated to amplify a 1,100 bp ppk1 fragment from Accumulibacter-like bacteria. Our results suggest that the Accumulibacter cluster has finer-scale architecture than previously revealed by 16S ribosomal RNA-based analyses.

Keywords

Enhanced biological phosphorus removal Activated sludge Rhodocyclus Accumulibacter phosphatis Polyphosphate kinase

Supplementary material

253_2007_1122_MOESM1_ESM.doc (784 kb)
ESM Polyphosphate kinase genes from full-scale activated sludge plants (DOC 802 kb)

Copyright information

© Springer-Verlag 2007