Applied Microbiology and Biotechnology

, Volume 76, Issue 5, pp 1093–1105

Comparative global transcription analysis of sodium hypochlorite, peracetic acid, and hydrogen peroxide on Pseudomonas aeruginosa

  • David A. Small
  • Wook Chang
  • Freshteh Toghrol
  • William E. Bentley
Genomics and Proteomics

DOI: 10.1007/s00253-007-1072-z

Cite this article as:
Small, D.A., Chang, W., Toghrol, F. et al. Appl Microbiol Biotechnol (2007) 76: 1093. doi:10.1007/s00253-007-1072-z

Abstract

Disinfectants are routinely used in hospitals and health care facilities for surface sterilization. However, the mechanisms by which these disinfectants kill and the extent to which bacteria, including Pseudomonas aeruginosa, are resistant remains unclear. Consequently, P. aeruginosa nosocomial infections result in considerable casualties and economic hardship. Previously, DNA microarrays were utilized to analyze the genome-wide transcription changes in P. aeruginosa after oxidative antimicrobial (sodium hypochlorite, peracetic acid, and hydrogen peroxide) exposure. Simultaneous analysis of these transcriptome datasets provided a comprehensive understanding of the differential responses to these disinfectants. An analysis of variance, functional classification analysis, metabolic pathway analysis, Venn diagram analysis, and principal component analysis revealed that sodium hypochlorite exposure resulted in more genome-wide changes than either peracetic acid or hydrogen peroxide exposures.

Keywords

Pseudomonas aeruginosa Sodium hypochlorite Peracetic acid Hydrogen peroxide Microarrays 

Supplementary material

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • David A. Small
    • 1
  • Wook Chang
    • 1
  • Freshteh Toghrol
    • 2
  • William E. Bentley
    • 1
  1. 1.Center for Biosystems ResearchUniversity of Maryland Biotechnology InstituteCollege ParkUSA
  2. 2.Microarray Research Laboratory, Biological and Economic Analysis Division, Office of Pesticide ProgramsUS Environmental Protection AgencyFort MeadeUSA
  3. 3.School of Chemical and Biomedical EngineeringNanyang Technological UniversitySingaporeSingapore

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