Applied Microbiology and Biotechnology

, Volume 75, Issue 5, pp 1173–1182

Anaerobic growth and potential for amino acid production by nitrate respiration in Corynebacterium glutamicum

Authors

  • Seiki Takeno
    • Department of Bioscience and Biotechnology, Faculty of AgricultureShinshu University
  • Junko Ohnishi
    • BioFrontier LaboratoriesKyowa Hakko Kogyo Co., Ltd.
  • Tomoha Komatsu
    • Department of Bioscience and Biotechnology, Faculty of AgricultureShinshu University
  • Tatsuya Masaki
    • Department of Bioscience and Biotechnology, Faculty of AgricultureShinshu University
  • Kikuo Sen
    • Department of Bioscience and Biotechnology, Faculty of AgricultureShinshu University
    • Department of Bioscience and Biotechnology, Faculty of AgricultureShinshu University
Applied Microbial and Cell Physiology

DOI: 10.1007/s00253-007-0926-8

Cite this article as:
Takeno, S., Ohnishi, J., Komatsu, T. et al. Appl Microbiol Biotechnol (2007) 75: 1173. doi:10.1007/s00253-007-0926-8

Abstract

Oxygen limitation is a crucial problem in amino acid fermentation by Corynebacterium glutamicum. Toward this subject, our study was initiated by analysis of the oxygen-requiring properties of C. glutamicum, generally regarded as a strict aerobe. This organism formed colonies on agar plates up to relatively low oxygen concentrations (0.5% O2), while no visible colonies were formed in the absence of O2. However, in the presence of nitrate (\({\text{NO}}^{ - }_{{\text{3}}} \)), the organism exhibited limited growth anaerobically with production of nitrite (\({\text{NO}}^{ - }_{{\text{2}}} \)), indicating that C. glutamicum can use nitrate as a final electron acceptor. Assays of cell extracts from aerobic and hypoxic cultures yielded comparable nitrate reductase activities, irrespective of nitrate levels. Genome analysis revealed a narK2GHJI cluster potentially relevant to nitrate reductase and transport. Disruptions of narG and narJ abolished the nitrate-dependent anaerobic growth with the loss of nitrate reductase activity. Disruption of the putative nitrate/nitrite antiporter gene narK2 did not affect the enzyme activity but impaired the anaerobic growth. These indicate that this locus is responsible for nitrate respiration. Agar piece assays using l-lysine- and l-arginine-producing strains showed that production of both amino acids occurred anaerobically by nitrate respiration, indicating the potential of C. glutamicum for anaerobic amino acid production.

Keywords

Corynebacterium glutamicum Oxygen limitation Nitrate reductase Nitrate respiration Amino acid production

Copyright information

© Springer-Verlag 2007