Applied Microbiology and Biotechnology

, Volume 74, Issue 5, pp 937–953

Towards industrial pentose-fermenting yeast strains

  • Bärbel Hahn-Hägerdal
  • Kaisa Karhumaa
  • César Fonseca
  • Isabel Spencer-Martins
  • Marie F. Gorwa-Grauslund
Mini-Review

DOI: 10.1007/s00253-006-0827-2

Cite this article as:
Hahn-Hägerdal, B., Karhumaa, K., Fonseca, C. et al. Appl Microbiol Biotechnol (2007) 74: 937. doi:10.1007/s00253-006-0827-2

Abstract

Production of bioethanol from forest and agricultural products requires a fermenting organism that converts all types of sugars in the raw material to ethanol in high yield and with a high rate. This review summarizes recent research aiming at developing industrial strains of Saccharomyces cerevisiae with the ability to ferment all lignocellulose-derived sugars. The properties required from the industrial yeast strains are discussed in relation to four benchmarks: (1) process water economy, (2) inhibitor tolerance, (3) ethanol yield, and (4) specific ethanol productivity. Of particular importance is the tolerance of the fermenting organism to fermentation inhibitors formed during fractionation/pretreatment and hydrolysis of the raw material, which necessitates the use of robust industrial strain background. While numerous metabolic engineering strategies have been developed in laboratory yeast strains, only a few approaches have been realized in industrial strains. The fermentation performance of the existing industrial pentose-fermenting S. cerevisiae strains in lignocellulose hydrolysate is reviewed. Ethanol yields of more than 0.4 g ethanol/g sugar have been achieved with several xylose-fermenting industrial strains such as TMB 3400, TMB 3006, and 424A(LNF-ST), carrying the heterologous xylose utilization pathway consisting of xylose reductase and xylitol dehydrogenase, which demonstrates the potential of pentose fermentation in improving lignocellulosic ethanol production.

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Bärbel Hahn-Hägerdal
    • 1
  • Kaisa Karhumaa
    • 1
  • César Fonseca
    • 2
  • Isabel Spencer-Martins
    • 2
  • Marie F. Gorwa-Grauslund
    • 1
  1. 1.Department of Applied MicrobiologyLund UniversityLundSweden
  2. 2.Centro de Recursos Microbiológicos (CREM), Biotechnology Unit, Faculty of Sciences and TechnologyNew University of LisbonCaparicaPortugal