Environmental Biotechnology

Applied Microbiology and Biotechnology

, Volume 75, Issue 1, pp 175-186

First online:

Influence of growth medium on cometabolic degradation of polycyclic aromatic hydrocarbons by Sphingomonas sp. strain PheB4

  • Yin ZhongAffiliated withState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityFutian-CityU Mangrove Research & Development Centre, Futian National Nature Reserve
  • , Tiangang LuanAffiliated withState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University Email author 
  • , Xiaowei WangAffiliated withState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityFutian-CityU Mangrove Research & Development Centre, Futian National Nature Reserve
  • , Chongyu LanAffiliated withState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University
  • , Nora F. Y. TamAffiliated withFutian-CityU Mangrove Research & Development Centre, Futian National Nature ReserveDepartment of Biology and Chemistry, City University of Hong Kong Email author 

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Abstract

The influence of growth medium on cometabolic degradation of polycyclic aromatic hydrocarbons (PAHs) was investigated when Sphingomonas sp. strain PheB4 isolated from surface mangrove sediments was grown in either phenanthrene-containing mineral salts medium (PMSM) or nutrient broth (NB). The NB-grown culture exhibited a more rapid cometabolic degradation of single and mixed non-growth substrate PAHs compared to the PMSM-grown culture. The concentrations of PAH metabolites were also lower in NB-grown culture than in PMSM-grown culture, suggesting that NB-grown culture removed metabolites at a faster rate, particularly, for metabolites produced from cometabolic degradation of a binary mixture of PAHs. Cometabolic pathways of single PAH (anthracene, fluorene, or fluoranthene) in NB-grown culture showed similarity to that in PMSM-grown culture. However, cometabolic pathways of mixed PAHs were more diverse in NB-grown culture than that in PMSM-grown culture. These results indicated that nutrient rich medium was effective in enhancing cometabolic degradation of mixed PAHs concomitant with a rapid removal of metabolites, which could be useful for the bioremediation of mixed PAHs contaminated sites using Sphingomonas sp. strain PheB4.

Keywords

Biodegradation Cometabolism Solid phase microextraction Mangrove ecosystems