Applied Microbiology and Biotechnology

, Volume 75, Issue 1, pp 175–186

Influence of growth medium on cometabolic degradation of polycyclic aromatic hydrocarbons by Sphingomonas sp. strain PheB4

Environmental Biotechnology

DOI: 10.1007/s00253-006-0789-4

Cite this article as:
Zhong, Y., Luan, T., Wang, X. et al. Appl Microbiol Biotechnol (2007) 75: 175. doi:10.1007/s00253-006-0789-4
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Abstract

The influence of growth medium on cometabolic degradation of polycyclic aromatic hydrocarbons (PAHs) was investigated when Sphingomonas sp. strain PheB4 isolated from surface mangrove sediments was grown in either phenanthrene-containing mineral salts medium (PMSM) or nutrient broth (NB). The NB-grown culture exhibited a more rapid cometabolic degradation of single and mixed non-growth substrate PAHs compared to the PMSM-grown culture. The concentrations of PAH metabolites were also lower in NB-grown culture than in PMSM-grown culture, suggesting that NB-grown culture removed metabolites at a faster rate, particularly, for metabolites produced from cometabolic degradation of a binary mixture of PAHs. Cometabolic pathways of single PAH (anthracene, fluorene, or fluoranthene) in NB-grown culture showed similarity to that in PMSM-grown culture. However, cometabolic pathways of mixed PAHs were more diverse in NB-grown culture than that in PMSM-grown culture. These results indicated that nutrient rich medium was effective in enhancing cometabolic degradation of mixed PAHs concomitant with a rapid removal of metabolites, which could be useful for the bioremediation of mixed PAHs contaminated sites using Sphingomonas sp. strain PheB4.

Keywords

BiodegradationCometabolismSolid phase microextractionMangrove ecosystems

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.State Key Laboratory of Biocontrol, School of Life SciencesSun Yat-Sen UniversityGuangzhouPeople’s Republic of China
  2. 2.Futian-CityU Mangrove Research & Development CentreFutian National Nature ReserveShenzhenPeople’s Republic of China
  3. 3.Department of Biology and ChemistryCity University of Hong KongKowloonPeople’s Republic of China