Applied Microbiology and Biotechnology

, Volume 74, Issue 2, pp 282–289

Thymidyl biosynthesis enzymes as antibiotic targets

  • Anatoly Chernyshev
  • Todd Fleischmann
  • Amnon Kohen
Mini-Review

DOI: 10.1007/s00253-006-0763-1

Cite this article as:
Chernyshev, A., Fleischmann, T. & Kohen, A. Appl Microbiol Biotechnol (2007) 74: 282. doi:10.1007/s00253-006-0763-1

Abstract

The two long-known “classical” enzymes of uridyl-5-methylation, thymidylate synthase and ribothymidyl synthase, have been joined by two alternative methylation enzymes, flavin-dependent thymidylate synthase and folate-dependent ribothymidyl synthase. These two newly discovered enzymes have much in common: both contain flavin cofactors, utilize methylenetetrahydrofolate as a source of methyl group, and perform thymidylate synthesis via chemical pathways distinct from those of their classic counterparts. Several severe human pathogens (e.g., typhus, anthrax, tuberculosis, and more) depend on these “alternative” enzymes for reproduction. These and other distinctive properties make the alternative enzymes and their corresponding genes appealing targets for new antibiotics.

Keywords

ThymineBiosynthesisFlavinThymidylate Synthase

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Anatoly Chernyshev
    • 1
  • Todd Fleischmann
    • 1
  • Amnon Kohen
    • 1
  1. 1.Department of ChemistryUniversity of IowaIowa CityUSA