Applied Microbiology and Biotechnology

, Volume 74, Issue 4, pp 851–856

Effect of surfactants on fluoranthene degradation by Pseudomonas alcaligenes PA-10

  • Anne Marie Hickey
  • Linda Gordon
  • Alan D. W. Dobson
  • Catherine T. Kelly
  • Evelyn M. Doyle
Applied Microbial and Cell Physiology

DOI: 10.1007/s00253-006-0719-5

Cite this article as:
Hickey, A.M., Gordon, L., Dobson, A.D.W. et al. Appl Microbiol Biotechnol (2007) 74: 851. doi:10.1007/s00253-006-0719-5

Abstract

Two surfactants, Tween 80 and JBR, were investigated for their effect on fluoranthene degradation by a Pseudomonad. Both surfactants enhanced fluoranthene degradation by Pseudomonas alcaligenes PA-10 in shake flask culture. This bacterium was capable of utilising the synthetic surfactant and the biosurfactant as growth substrates and the critical micelle concentration of neither compound inhibited bacterial growth. The biosurfactant JBR significantly increased polycyclic aromatic hydrocarbon (PAH) desorption from soil. Inoculation of fluoranthene-contaminated soil microcosms with P. alcaligenes PA-10 resulted in the removal of significant amounts (45 ± 5%) of the PAH after 28 days compared to an uninoculated control. Addition of the biosurfactant increased the initial rate of fluoranthene degradation in the inoculated microcosm. The presence of a lower molecular weight PAH, phenanthrene, had a similar effect on the rate of fluoranthene removal.

Keywords

PAHsFluorantheneBiodegradationSurfactants

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Anne Marie Hickey
    • 1
  • Linda Gordon
    • 2
  • Alan D. W. Dobson
    • 2
  • Catherine T. Kelly
    • 1
  • Evelyn M. Doyle
    • 1
  1. 1.School of Biology and Environmental ScienceUniversity College DublinDublin 4Republic of Ireland
  2. 2.Department of MicrobiologyNational University of Ireland, University College CorkCorkRepublic of Ireland