Applied Microbiology and Biotechnology

, Volume 74, Issue 1, pp 176–185

Toxicogenomic analysis of sodium hypochlorite antimicrobial mechanisms in Pseudomonas aeruginosa

  • David A. Small
  • Wook Chang
  • Freshteh Toghrol
  • William E. Bentley
Genomics and Proteomics

DOI: 10.1007/s00253-006-0644-7

Cite this article as:
Small, D.A., Chang, W., Toghrol, F. et al. Appl Microbiol Biotechnol (2007) 74: 176. doi:10.1007/s00253-006-0644-7

Abstract

Sodium hypochlorite (bleach) is routinely used in hospitals and health care facilities for surface sterilization; however, the mechanism of action by which this disinfectant kills and the extent to which Pseudomonas aeruginosa is resistant to sodium hypochlorite have not been elucidated. Consequently, nosocomial infections from P. aeruginosa result in considerable casualties and economic hardship. We report the genome-wide transcriptome response of P. aeruginosa to sodium hypochlorite-induced oxidative stress via the use of DNA microarrays. In addition to a general oxidative stress response, our data revealed a downregulation of virtually all genes related to oxidative phosphorylation and electron transport and an upregulation of many organic sulfur transport and metabolism genes.

Keywords

AntimicrobialToxicogenomicMicroarray

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • David A. Small
    • 1
  • Wook Chang
    • 1
  • Freshteh Toghrol
    • 2
  • William E. Bentley
    • 1
  1. 1.Center for Biosystems ResearchUniversity of Maryland Biotechnology InstituteCollege ParkUSA
  2. 2.Microarray Research Laboratory, Biological and Economic Analysis Division , Office of Pesticide ProgramsU. S. Environmental Protection AgencyFort MeadeUSA