Applied Microbiology and Biotechnology

, Volume 73, Issue 3, pp 618–630

A cel44C-man26A gene of endophytic Paenibacillus polymyxa GS01 has multi-glycosyl hydrolases in two catalytic domains

  • Kye Man Cho
  • Su Young Hong
  • Sun Mi Lee
  • Yong Hee Kim
  • Goon Gjung Kahng
  • Hoon Kim
  • Han Dae Yun
Biotechnologically Relevant Enzymes and Proteins

DOI: 10.1007/s00253-006-0523-2

Cite this article as:
Cho, K.M., Hong, S.Y., Lee, S.M. et al. Appl Microbiol Biotechnol (2006) 73: 618. doi:10.1007/s00253-006-0523-2

Abstract

A bacterial strain Paenibacillus polymyxa GS01 was isolated from the interior of the roots of Korean cultivars of ginseng (Panax ginseng C. A. Meyer). The cel44C-man26A gene was cloned from this endophytic strain. This 4,056-bp gene encodes for a 1,352-aa protein which, based on BLAST search homologies, contains a glycosyl hydrolase family 44 (GH44) catalytic domain, a fibronectin domain type 3, a glycosyl hydrolase family 26 (GH26) catalytic domain, and a cellulose-binding module type 3. The multifunctional enzyme domain GH44 possesses cellulase, xylanase, and lichenase activities, while the enzyme domain GH26 possesses mannanase activity. The Cel44C enzyme expressed in and purified from Escherichia coli has an optimum pH of 7.0 for cellulase and lichenase activities, but is at an optimum pH of 5.0 for xylanase and mannanase activities. The optimum temperature for enzymatic activity was 50°C for all substrates. No detectable enzymatic activity was detected for the Cel44C-Man26A mutants E91A and E222A. These results suggest that the amino acid residues Glu91 and Glu222 may play an important role in the glycosyl hydrolases activity of Cel44C-Man26A.

Keywords

Paenibacillus polymyxa GS01 Glycosyl hydrolases Rhizobacterium 

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Kye Man Cho
    • 1
  • Su Young Hong
    • 1
  • Sun Mi Lee
    • 1
  • Yong Hee Kim
    • 1
  • Goon Gjung Kahng
    • 1
  • Hoon Kim
    • 3
  • Han Dae Yun
    • 2
  1. 1.Division of Applied Life ScienceGyeongsang National UniversityChinjuSouth Korea
  2. 2.Research Institute of Life ScienceGyeongsang National UniversityChinjuSouth Korea
  3. 3.Department of Agricultural ChemistrySunchon National UniversitySunchonSouth Korea

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