Applied Microbiology and Biotechnology

, Volume 73, Issue 3, pp 519–524

Antioxidants enhanced production of destruxin E from cultivation of Metarhizium anisopliae

  • Yerra Koteswara Rao
  • Chia-Heng Tsou
  • Yew-Min Tzeng
Biotechnological Products and Process Engineering

DOI: 10.1007/s00253-006-0493-4

Cite this article as:
Rao, Y.K., Tsou, C. & Tzeng, Y. Appl Microbiol Biotechnol (2006) 73: 519. doi:10.1007/s00253-006-0493-4

Abstract

The effect of antioxidants on the production of an important cyclohexadepsipeptide congener destruxin E (dtx E) was investigated using the entomopathogenic fungus Metarhizium anisopliae F061. In shaker flask cultivations, 0.015% of menadione-enhanced dtx E production of 220.4 mg/l compared to the control cultivation 90.2 mg/l, which was illustrated by stimulation of dtx E biosynthesis through two electron reduction DT-diaphorase processes in cultivation of M. anisopliae. In 5-l stirred-tank bioreactor cultivation with menadione addition and of control pH 4.0, a yield of 454.6 mg/l of dtx E was obtained after 7 days, and was nearly 30 and 15-fold higher than that from no pH control, and controlled pH 2.0 cultivations, respectively. Further cultivation in a 20-l airlift bioreactor, at pH 4.0, dtx E obtained on the 9th day was 406.0 mg/l, which was much higher than the standard cultivation of no pH control yield 203.3 mg/l on the 11th day. Thus, the present study provides useful information for enhancing dtx E production in cultivation.

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Yerra Koteswara Rao
    • 1
  • Chia-Heng Tsou
    • 1
  • Yew-Min Tzeng
    • 1
  1. 1.Institute of BiotechnologyChaoyang University of TechnologyWufengRepublic of China