Applied Microbiology and Biotechnology

, Volume 73, Issue 2, pp 394–403

Intraspecific diversity of Oenococcus oeni strains determined by sequence analysis of target genes

  • Arnaud Delaherche
  • Elisabeth Bon
  • Aurelien Dupé
  • Megumi Lucas
  • Benoit Arveiler
  • Antoine De Daruvar
  • Aline Lonvaud-Funel
Applied Genetics and Molecular Biotechnology

DOI: 10.1007/s00253-006-0487-2

Cite this article as:
Delaherche, A., Bon, E., Dupé, A. et al. Appl Microbiol Biotechnol (2006) 73: 394. doi:10.1007/s00253-006-0487-2

Abstract

Using molecular techniques and sequencing, we studied the intraspecific diversity of Oenococcus oeni, a lactic acid bacterium involved in red winemaking. A relationship between the phenotypic and genotypic characterization of 16 O. oeni strains isolated from wine with different levels of enological potential was shown. The study was based on the comparative genomic analysis by subtractive hybridization between two strains of O. oeni with opposite enological potential. The genomic sequences obtained from subtractive hybridization were amplified by polymerase chain reaction and sequenced for the 16 strains. A considerable diversity among strains of O. oeni was observed.

Keywords

O. oeni speciesVariabilityGenes

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Arnaud Delaherche
    • 1
  • Elisabeth Bon
    • 2
  • Aurelien Dupé
    • 1
  • Megumi Lucas
    • 1
  • Benoit Arveiler
    • 3
  • Antoine De Daruvar
    • 2
  • Aline Lonvaud-Funel
    • 1
  1. 1.Faculte d’Oenologie, UMR 1219Oenologie-Ampelologie, INRA-Universite Victor Segalen Bordeaux 2TalenceFrance
  2. 2.CBiB-Centre de Bioinformatique de BordeauxUniversite Victor Segalen Bordeaux 2BordeauxFrance
  3. 3.Laboratoire de Pathologie Moleculaire et Therapie Genique, UMR CNRS 5541Universite Victor Segalen Bordeaux 2BordeauxFrance