Applied Microbiology and Biotechnology

, Volume 69, Issue 2, pp 170–177

The solvent-tolerant Pseudomonas putida S12 as host for the production of cinnamic acid from glucose

  • Karin Nijkamp
  • Nicole van Luijk
  • Jan A. M. de Bont
  • Jan Wery
Applied Genetics and Molecular Biotechnology

DOI: 10.1007/s00253-005-1973-7

Cite this article as:
Nijkamp, K., van Luijk, N., de Bont, J.A.M. et al. Appl Microbiol Biotechnol (2005) 69: 170. doi:10.1007/s00253-005-1973-7

Abstract

A Pseudomonas putida S12 strain was constructed that efficiently produced the fine chemical cinnamic acid from glucose or glycerol via the central metabolite phenylalanine. The gene encoding phenylalanine ammonia lyase from the yeast Rhodosporidium toruloides was introduced. Phenylalanine availability was the main bottleneck in cinnamic acid production, which could not be overcome by the overexpressing enzymes of the phenylalanine biosynthesis pathway. A successful approach in abolishing this limitation was the generation of a bank of random mutants and selection on the toxic phenylalanine anti-metabolite m-fluoro-phenylalanine. Following high-throughput screening, a mutant strain was obtained that, under optimised culture conditions, accumulated over 5 mM of cinnamic acid with a yield (Cmol%) of 6.7%.

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Karin Nijkamp
    • 1
    • 3
  • Nicole van Luijk
    • 2
  • Jan A. M. de Bont
    • 1
  • Jan Wery
    • 1
  1. 1.TNO Quality of LifeBusiness Unit Bioconversion and Processes for Food IndustryApeldoornThe Netherlands
  2. 2.TNO Quality of LifeBusiness Unit MicrobiologyZeistThe Netherlands
  3. 3.ApeldoornThe Netherlands