Applied Microbiology and Biotechnology

, Volume 68, Issue 3, pp 412–416

Synthesis of prebiotic galactooligosaccharides using whole cells of a novel strain, Bifidobacterium bifidum NCIMB 41171

  • George Tzortzis
  • Athanasios K. Goulas
  • Glenn R. Gibson
Applied Microbial and Cell Physiology

DOI: 10.1007/s00253-005-1919-0

Cite this article as:
Tzortzis, G., Goulas, A.K. & Gibson, G.R. Appl Microbiol Biotechnol (2005) 68: 412. doi:10.1007/s00253-005-1919-0

Abstract

A novel strain of Bifidobacterium bifidum NCIMB 41171, isolated from a faecal sample from a healthy human volunteer and able to express β-galactosidase activity, was used in synthesis reactions for the production of galactooligosaccharide from lactose. The β-galactosidase activity of whole bifidobacterial cells showed an optimum activity at pH 6.8–7.0 and 40°C. The transgalactosylation activity of the B. bifidum cells from 50% (w/w) lactose resulted in a galactooligosaccharide mixture (20% w/w) comprising (w/w): 25% disaccharides, 35% trisaccharides, 25% tetrasaccharides and 15% pentasaccharides. Using different initial lactose concentrations, the conversion rate to galactooligosaccharides was maximum (35%) when 55% (w/w) lactose was used. In fermentation experiments, B. bifidum showed an increased preference towards the produced galactooligosaccharide mixture, displaying higher growth rate and short-chain fatty acid production when compared with commercially available oligosaccharides.

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • George Tzortzis
    • 1
  • Athanasios K. Goulas
    • 1
  • Glenn R. Gibson
    • 1
  1. 1.School of Food BiosciencesThe University of ReadingReadingUK