Applied Microbiology and Biotechnology

, Volume 72, Issue 3, pp 521–528

Development of an expression vector for Metarhizium anisopliae based on the tef-1α homologous promoter

  • Luciano Nakazato
  • Valéria Dutra
  • Leonardo Broetto
  • Charley Christian Staats
  • Marilene Henning Vainstein
  • Augusto Schrank
Applied Genetics and Molecular Biotechnology

DOI: 10.1007/s00253-005-0292-3

Cite this article as:
Nakazato, L., Dutra, V., Broetto, L. et al. Appl Microbiol Biotechnol (2006) 72: 521. doi:10.1007/s00253-005-0292-3

Abstract

The high-conserved translation elongation factor 1 α (tef-1α) gene from the enthomopathogenic fungus Metarhizium anisopliae was characterized to select the promoter region. A 640-bp DNA fragment upstream to the start codon was employed to drive the expression of the reporter protein sGFP or a dominant selectable marker, the gene bar (resistance to ammonium glufosinate). Transformants carrying this homologous promoter system showed no difference in virulence bioassays against the cattle tick Boophilus microplus comparing to the M. anisopliae wild-type strain. Moreover, GFP fluorescence was detected during tick infection bioassay.

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Luciano Nakazato
    • 1
  • Valéria Dutra
    • 1
  • Leonardo Broetto
    • 1
  • Charley Christian Staats
    • 1
  • Marilene Henning Vainstein
    • 1
  • Augusto Schrank
    • 1
    • 2
  1. 1.Centro de BiotecnologiaUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  2. 2.Departamento de Biologia Molecular e BiotecnologiaUniversidade Federal do Rio Grande do SulPorto AlegreBrazil

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