Applied Microbiology and Biotechnology

, Volume 71, Issue 4, pp 440–443

An aqueous–organic two-phase bioprocess for efficient production of the natural aroma chemicals 2-phenylethanol and 2-phenylethylacetate with yeast

Biotechnological Products and Process Engineering

DOI: 10.1007/s00253-005-0281-6

Cite this article as:
Etschmann, M.M.W. & Schrader, J. Appl Microbiol Biotechnol (2006) 71: 440. doi:10.1007/s00253-005-0281-6


The natural aroma chemicals 2-phenylethanol (2-PE) and 2-phenylethylacetate (2-PEAc) are of high industrial relevance and can be produced from l-phenylalanine in a yeast-based process with growth-associated product formation. Due to product inhibition, in situ product removal is mandatory to obtain economically interesting concentrations. A fed-batch approach using polypropylene glycol 1200 as in situ extractant and the precursor in a saturated concentration led to the highest 2-PE productivity reported for a bioprocess so far. With Kluyveromyces marxianus CBS 600, 26.5 g/l 2-PE and 6.1 g/l 2-PEAc in the organic phase were obtained, corresponding to space–time yields of 0.33 and 0.08 g/l h, respectively.

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.Dechema e.V, Karl-Winnacker-Institut, Biochemical Engineering GroupFrankfurt am MainGermany