Applied Microbiology and Biotechnology

, Volume 70, Issue 4, pp 444–450

Purification and characterization of tannase from Paecilomyces variotii: hydrolysis of tannic acid using immobilized tannase

Authors

    • Department of Earth and Environmental SciencesKorea University
  • N. Raman
    • Centre for Advanced studies in BotanyUniversity of Madras
  • D.-J. Kim
    • Department of Earth and Environmental SciencesKorea University
Biotechnologically Relevant Enzymes and Proteins

DOI: 10.1007/s00253-005-0082-y

Cite this article as:
Mahendran, B., Raman, N. & Kim, D. Appl Microbiol Biotechnol (2006) 70: 444. doi:10.1007/s00253-005-0082-y

Abstract

An extracellular tannase (tannin acyl hydrolase) was isolated from Paecilomyces variotii and purified from cell-free culture filtrate using ammonium sulfate precipitation followed by ion exchange and gel filtration chromatography. Fractional precipitation of the culture filtrate with ammonium sulfate yielded 78.7% with 13.6-folds purification, and diethylaminoethyl–cellulose column chromatography and gel filtration showed 19.4-folds and 30.5-folds purifications, respectively. Molecular mass of tannase was found 149.8 kDa through native polyacrylamide gel electrophoresis (PAGE) analysis. Sodium dodecyl sulphate–PAGE revealed that the purified tannase was a monomeric enzyme with a molecular mass of 45 kDa. Temperature of 30 to 50°C and pH of 5.0 to 7.0 were optimum for tannase activity and stability. Tannase immobilized on alginate beads could hydrolyze tannic acid even after extensive reuse and retained about 85% of the initial activity. Thin layer chromatography, high performance liquid chromatography, and 1H-nuclear magnetic resonance spectral analysis confirmed that gallic acid was formed as a byproduct during hydrolysis of tannic acid.

Copyright information

© Springer-Verlag 2005