Applied Microbiology and Biotechnology

, Volume 69, Issue 6, pp 689–696

Direct mating between diploid sake strains of Saccharomyces cerevisiae

  • Shinji Hashimoto
  • Kazuo Aritomi
  • Takafumi Minohara
  • Yoshinori Nishizawa
  • Hisashi Hoshida
  • Susumu Kashiwagi
  • Rinji Akada
Applied Genetics and Molecular Biotechnology

DOI: 10.1007/s00253-005-0039-1

Cite this article as:
Hashimoto, S., Aritomi, K., Minohara, T. et al. Appl Microbiol Biotechnol (2006) 69: 689. doi:10.1007/s00253-005-0039-1

Abstract

Various auxotrophic mutants of diploid heterothallic Japanese sake strains of Saccharomyces cerevisiae were utilized for selecting mating-competent diploid isolates. The auxotrophic mutants were exposed to ultraviolet (UV) irradiation and crossed with laboratory haploid tester strains carrying complementary auxotrophic markers. Zygotes were then selected on minimal medium. Sake strains exhibiting a MATa or MATα mating type were easily obtained at high frequency without prior sporulation, suggesting that the UV irradiation induced homozygosity at the MAT locus. Flow cytometric analysis of a hybrid showed a twofold higher DNA content than the sake diploid parent, consistent with tetraploidy. By crossing strains of opposite mating type in all possible combinations, a number of hybrids were constructed. Hybrids formed in crosses between traditional sake strains and between a natural nonhaploid isolate and traditional sake strains displayed equivalent fermentation ability without any apparent defects and produced comparable or improved sake. Isolation of mating-competent auxotrophic mutants directly from industrial yeast strains allows crossbreeding to construct polyploids suitable for industrial use without dependence on sporulation.

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Shinji Hashimoto
    • 1
    • 3
  • Kazuo Aritomi
    • 2
  • Takafumi Minohara
    • 1
  • Yoshinori Nishizawa
    • 1
  • Hisashi Hoshida
    • 1
  • Susumu Kashiwagi
    • 3
  • Rinji Akada
    • 1
  1. 1.Department of Applied Chemistry and Chemical Engineering, Faculty of EngineeringYamaguchi UniversityUbeJapan
  2. 2.Research and Development DepartmentSanwa Kagaku Kenkyusho Co. Ltd.Inabe-shi MieJapan
  3. 3.Yamaguchi Prefectural Industrial Technology InstituteUbeJapan

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