Applied Microbiology and Biotechnology

, Volume 67, Issue 6, pp 807–815

Examination of fungal stress response genes using Saccharomyces cerevisiae as a model system: targeting genes affecting aflatoxin biosynthesis by Aspergillus flavus Link

  • Jong H. Kim
  • Bruce C. Campbell
  • Jiujiang Yu
  • Noreen Mahoney
  • Kathleen L. Chan
  • Russell J. Molyneux
  • Deepak Bhatnagar
  • Thomas E. Cleveland
Applied Genetics and Molecular Biotechnology

DOI: 10.1007/s00253-004-1821-1

Cite this article as:
Kim, J.H., Campbell, B.C., Yu, J. et al. Appl Microbiol Biotechnol (2005) 67: 807. doi:10.1007/s00253-004-1821-1

Abstract

Saccharomyces cerevisiae served as a model fungal system to examine functional genomics of oxidative stress responses and reactions to test antioxidant compounds. Twenty-two strains of S. cerevisiae, including a broad spectrum of singular gene deletion mutants, were exposed to hydrogen peroxide (H2O2) to examine phenotypic response to oxidative stress. Responses of particular mutants treated with gallic, tannic or caffeic acids, or methyl gallate, during H2O2 exposure, indicated that these compounds alleviated oxidative stress. These compounds are also potent inhibitors of aflatoxin biosynthesis in Aspergillus flavus. To gain further insights into a potential link between oxidative stress and aflatoxin biosynthesis, 43 orthologs of S. cerevisiae genes involved in gene regulation, signal transduction (e.g., SHO1, HOG1, etc.) and antioxidation (e.g., CTT1, CTA1, etc.) were identified in an A. flavus expressed sequence tag library. A successful exemplary functional complementation of an antioxidative stress gene from A. flavus, mitochondrial superoxide dismutase (sodA), in a sod2Δ yeast mutant further supported the potential of S. cerevisiae deletion mutants to serve as a model system to study A. flavus. Use of this system to further examine functional genomics of oxidative stress in aflatoxigenesis and reduction of aflatoxin biosynthesis by antioxidants is discussed.

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Jong H. Kim
    • 1
  • Bruce C. Campbell
    • 1
  • Jiujiang Yu
    • 2
  • Noreen Mahoney
    • 1
  • Kathleen L. Chan
    • 1
  • Russell J. Molyneux
    • 1
  • Deepak Bhatnagar
    • 2
  • Thomas E. Cleveland
    • 2
  1. 1.Plant Mycotoxin Research UnitWestern Regional Research Center, USDA-ARSAlbanyUSA
  2. 2.Food and Feed Safety Research UnitSouthern Regional Research Center, USDA-ARSNew OrleansUSA