Applied Microbiology and Biotechnology

, Volume 67, Issue 5, pp 679–683

Bile acids are new products of a marine bacterium, Myroides sp. strain SM1

  • Suppasil Maneerat
  • Teruhiko Nitoda
  • Hiroshi Kanzaki
  • Fusako Kawai
Applied Microbial and Cell Physiology

DOI: 10.1007/s00253-004-1777-1

Cite this article as:
Maneerat, S., Nitoda, T., Kanzaki, H. et al. Appl Microbiol Biotechnol (2005) 67: 679. doi:10.1007/s00253-004-1777-1

Abstract

Strain SM1 was isolated as a biosurfactant-producing microorganism from seawater and presumptively identified as Myroides sp., based on morphology, biochemical characteristics and 16S rDNA sequence. The strain produced surface-active compounds in marine broth, which were purified, using emulsification activity for n-hexadecane as an indicator. The purified compounds were identified by thin-layer chromatography, 1H- and 13C-NMR spectra and fast atom bombardment mass spectrometry as cholic acid, deoxycholic acid and their glycine conjugates. Type strains of the genus Myroides, M. odoratus JCM7458 and M. odoramitimus JCM7460, also produced these compounds. Myroides sp. strain SM1 possessed a biosynthetic route to cholic acid from cholesterol. Thus, bile acids were found as new products of prokaryotic cells, genus Myroides.

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Suppasil Maneerat
    • 1
  • Teruhiko Nitoda
    • 2
  • Hiroshi Kanzaki
    • 2
  • Fusako Kawai
    • 1
  1. 1.Laboratory of Applied Microbiology, Research Institute for BioresourcesOkayama UniversityKurashikiJapan
  2. 2.Laboratory of Bioresources Chemistry, Faculty of AgricultureOkayama UniversityOkayamaJapan