Applied Microbiology and Biotechnology

, Volume 66, Issue 3, pp 291–296

Translation efficiency mediated by the 5′ untranslated region greatly affects protein production in Aspergillus oryzae

  • Akio Koda
  • Toshitaka Minetoki
  • Kenji Ozeki
  • Masato Hirotsune
Applied Genetics and Molecular Biotechnology

DOI: 10.1007/s00253-004-1681-8

Cite this article as:
Koda, A., Minetoki, T., Ozeki, K. et al. Appl Microbiol Biotechnol (2004) 66: 291. doi:10.1007/s00253-004-1681-8

Abstract

We demonstrate that the 5′ untranslated region (5′UTR) plays an important role in determining translation efficiency in Aspergillus oryzae, using a model β-glucuronidase (GUS) expression system. Alterations in the 5′UTR resulted in an increase in GUS activity of up to eight-fold, without affecting mRNA levels. Moreover, using the most effective 5′UTR construct, we could achieve remarkable intracellular overproduction of GUS protein; and the GUS level reached more than 50% of the total soluble protein. This is the first experimental evidence indicating the feasibility of improving recombinant protein yield by promoting translation initiation in filamentous fungi.

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Akio Koda
    • 1
  • Toshitaka Minetoki
    • 1
  • Kenji Ozeki
    • 1
  • Masato Hirotsune
    • 1
  1. 1.General Research LaboratoryOzeki CorporationHyogoJapan