Abstract
Cerein 8A is a bacteriocin produced by the soil bacterium Bacillus cereus 8A, isolated from native woodlands of Brazil. The influence of temperature and media on the growth of B. cereus 8A and the production of this bacteriocin was studied during batch cultivation. Maximum activity was detected by cultivation in brain/heart infusion broth, reaching 3200 activity units ml−1. Bacteriocin was also produced in peptone, MRS, Mueller–Hinton and nutrient broth, while no activity was observed during cultivation in thioglycollate or tryptic soy broth. Temperature had a strong influence on bacteriocin production, which was higher at 30 °C than at 25 °C. An important decrease in bacteriocin activity was observed at 37 °C. The relationship between growth and specific production rates, as a function of the temperature, showed different kinetics of production and there were several peaks in the specific production rates during growth. Bacteriocin was produced at the stationary phase, indicating it is synthesized as a secondary metabolite.
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This work received financial support from Conselho Nacional de Desenvolvimento Científico e Tecnologico, Brazil.
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Bizani, D., Brandelli, A. Influence of media and temperature on bacteriocin production by Bacillus cereus 8A during batch cultivation. Appl Microbiol Biotechnol 65, 158–162 (2004). https://doi.org/10.1007/s00253-004-1570-1
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DOI: https://doi.org/10.1007/s00253-004-1570-1