Applied Microbiology and Biotechnology

, Volume 65, Issue 1, pp 110–118

Molecular and physiological characterisation of a 3-phytase from soil bacterium Klebsiella sp. ASR1

  • A. Sajidan
  • A. Farouk
  • R. Greiner
  • P. Jungblut
  • E.-C. Müller
  • R. Borriss
Original Paper

DOI: 10.1007/s00253-003-1530-1

Cite this article as:
Sajidan, A., Farouk, A., Greiner, R. et al. Appl Microbiol Biotechnol (2004) 65: 110. doi:10.1007/s00253-003-1530-1

Abstract

Klebsiella sp. strain ASR1 isolated from an Indonesian rice field is able to hydrolyse myo-inositol hexakis phosphate (phytate). The phytase protein was purified and characterised as a 42 kDa protein accepting phytate, NADP and sugar phosphates as substrates. The corresponding gene (phyK) was cloned from chromosomal DNA using a combined approach of protein and genome analysis, and expressed in Escherichia coli. The recombinant enzyme was identified as a 3-phytase yielding myo-inositol monophosphate, Ins(2)P, as the final product of enzymatic phytate hydrolysis. Based on its amino acid sequence, PhyK appears to be a member of a hitherto unknown subfamily of histidine acid phytate-degrading enzymes with the active site RHGXRXP and HD sequence motifs, and is different from other general phosphatases and phytases. Due to its ability to degrade sodium phytate to the mono phosphate ester, the phyK gene product is an interesting candidate for industrial and agricultural applications to make phytate phosphorous available for plant and animal nutrition.

Supplementary material

Supplementary Material

supp.pdf (361 kb)
(PDF 361 KB)

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • A. Sajidan
    • 1
  • A. Farouk
    • 1
  • R. Greiner
    • 2
  • P. Jungblut
    • 3
  • E.-C. Müller
    • 3
  • R. Borriss
    • 1
  1. 1.Department of Bacterial Genetics, Institute of BiologyHumboldt UniversityBerlinGermany
  2. 2.Centre for Molecular BiologyFederal Research Centre for NutritionKarlsruheGermany
  3. 3.Max Delbrück Centre for Molecular MedicineBerlinGermany

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