Applied Microbiology and Biotechnology

, Volume 63, Issue 2, pp 143–146

Microbial fed-batch production of 1,3-propanediol by Klebsiella pneumoniae under micro-aerobic conditions

  • X. Chen
  • D.-J. Zhang
  • W.-T. Qi
  • S.-J. Gao
  • Z.-L. Xiu
  • P. Xu
Short Contribution

DOI: 10.1007/s00253-003-1369-5

Cite this article as:
Chen, X., Zhang, DJ., Qi, WT. et al. Appl Microbiol Biotechnol (2003) 63: 143. doi:10.1007/s00253-003-1369-5

Abstract

The microbial production of 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae under micro-aerobic conditions was investigated in this study. The experimental results of batch fermentation showed that the final concentration and yield of 1,3-PD on glycerol under micro-aerobic conditions approached values achieved under anaerobic conditions. However, less ethanol was produced under microaerobic than anaerobic conditions at the end of fermentation. The batch micro-aerobic fermentation time was markedly shorter than that of anaerobic fermentation. This led to an increment of productivity of 1,3-PD. For instance, the concentration, molar yield, and productivity of 1,3-PD of batch micro-aerobic fermentation by K. pneumoniae DSM 2026 were 17.65 g/l, 56.13%, and 2.94 g l−1 h−1, respectively, with a fermentation time of 6 h and an initial glycerol concentration of 40 g/l. Compared with DSM 2026, the microbial growth of K. pneumoniae AS 1.1736 was slow and the concentration of 1,3-PD was low under the same conditions. Furthermore, the microbial growth in fed-batch fermentation by K. pneumoniae DSM 2026 was faster under micro-aerobic than anaerobic conditions. The concentration, molar yield, and productivity of 1,3-PD in fed-batch fermentation under micro-aerobic conditions were 59.50 g/l, 51.75%, and 1.57 g l−1 h−1, respectively. The volumetric productivity of 1,3-PD under microaerobic conditions was almost twice that of anaerobic fed-batch fermentation, at 1.57 and 0.80 g l−1 h−1, respectively.

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • X. Chen
    • 1
  • D.-J. Zhang
    • 1
  • W.-T. Qi
    • 1
  • S.-J. Gao
    • 1
  • Z.-L. Xiu
    • 1
  • P. Xu
    • 2
  1. 1.Department of BiotechnologyDalian University of TechnologyDalian P. R. China
  2. 2.State Key Laboratory of Microbial TechnologyShandong UniversityJinan P. R. China