Applied Microbiology and Biotechnology

, Volume 59, Issue 2, pp 329–331

An arming yeast with the ability to entrap fluorescent 17β-estradiol on the cell surface

  •  M. Yasui
  •  S. Shibasaki
  •  K. Kuroda
  •  M. Ueda
  •  N. Kawada
  •  J. Nishikawa
  •  T. Nishihara
  •  A. Tanaka
Short Contribution

DOI: 10.1007/s00253-002-1019-3

Cite this article as:
Yasui, M., Shibasaki, S., Kuroda, K. et al. Appl Microbiol Biotechnol (2002) 59: 329. doi:10.1007/s00253-002-1019-3

Abstract.

We constructed a novel surface-engineered yeast displaying the ligand-binding domain of the rat estrogen receptor (ERLBD). ERLBD, display of which on the yeast cell surface was confirmed by immunofluorescence, possessed strong binding activity to fluorescent 17β-estradiol – an analogue of the natural ligand of the estrogen receptor – that was comparable to the activity of the native receptor. Environmental homeostasis has recently been disturbed by endocrine disruptors, which cause confusion in the hormone secretion system. It is therefore very important to identify chemical compounds with hormone-like activity and remove them from the environment. The present results demonstrate that the new arming yeast displaying ERLBD on its cell surface will be capable of screening, entrapping, and removing estradiol-like compounds from the environment.

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  •  M. Yasui
    • 1
  •  S. Shibasaki
    • 1
  •  K. Kuroda
    • 1
  •  M. Ueda
    • 1
  •  N. Kawada
    • 2
  •  J. Nishikawa
    • 3
  •  T. Nishihara
    • 3
  •  A. Tanaka
    • 1
  1. 1.Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606–8501, Japan
  2. 2.Daicel Chemical Industries Ltd., Shinzaike, Aboshi-ku, Himeji, Hyogo 671–1283, Japan
  3. 3.Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical Sciences, Osaka University, 1–6 Yamada-oka, Suita, Osaka 565–0871, Japan

Personalised recommendations