Microbial diversity of a mesophilic hydrogen-producing sludge
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- Fang, H., Zhang, T. & Liu, H. Appl Microbiol Biotechnol (2002) 58: 112. doi:10.1007/s00253-001-0865-8
A hydrogen-producing sludge degraded 99% of glucose at 36 °C and pH 5.5, producing a methane-free biogas (comprising 64% hydrogen) and an effluent comprising mostly butyrate, acetate, and ethanol. The yield was 0.26 l H2 g–1 glucose and the production rate per gram of volatile suspended solids was 4.6 l H2 day–1. A 16S rDNA library was constructed from the sludge for microbial species determination. A total of 96 clones were selected for plasmids recovery, screened by denaturing gradient gel electrophoresis, and sequenced for rDNA. Based on the phylogenetic analysis of the rDNA sequences, 64.6% of all the clones were affiliated with three Clostridium species (Clostridiaceae), 18.8% with Enterobacteriaceae, and 3.1% with Streptococcus bovis (Streptococcaceae). The remaining 13.5% belonged to eight operational taxonomic units, the affiliations of which were not identified.