Applied Microbiology and Biotechnology

, Volume 58, Issue 1, pp 112–118

Microbial diversity of a mesophilic hydrogen-producing sludge

  •  H. Fang
  •  T. Zhang
  •  H. Liu
Original Paper

DOI: 10.1007/s00253-001-0865-8

Cite this article as:
Fang, H., Zhang, T. & Liu, H. Appl Microbiol Biotechnol (2002) 58: 112. doi:10.1007/s00253-001-0865-8

Abstract.

A hydrogen-producing sludge degraded 99% of glucose at 36 °C and pH 5.5, producing a methane-free biogas (comprising 64% hydrogen) and an effluent comprising mostly butyrate, acetate, and ethanol. The yield was 0.26 l H2 g–1 glucose and the production rate per gram of volatile suspended solids was 4.6 l H2 day–1. A 16S rDNA library was constructed from the sludge for microbial species determination. A total of 96 clones were selected for plasmids recovery, screened by denaturing gradient gel electrophoresis, and sequenced for rDNA. Based on the phylogenetic analysis of the rDNA sequences, 64.6% of all the clones were affiliated with three Clostridium species (Clostridiaceae), 18.8% with Enterobacteriaceae, and 3.1% with Streptococcus bovis (Streptococcaceae). The remaining 13.5% belonged to eight operational taxonomic units, the affiliations of which were not identified.

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  H. Fang
    • 1
  •  T. Zhang
    • 1
  •  H. Liu
    • 1
  1. 1.Centre for Environmental Engineering Research, Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China