Immunogenetics

, Volume 50, Issue 1, pp 60–66

Identification and characterization of a fish natural resistance-associated macrophage protein (NRAMP) cDNA

  • Jeroen P. J. Saeij
  • G. F. Wiegertjes
  • René J. M. Stet
ORIGINAL PAPER

DOI: 10.1007/s002510050686

Cite this article as:
Saeij, J., Wiegertjes, G. & Stet, R. Immunogenetics (1999) 50: 60. doi:10.1007/s002510050686

Abstract

 The mouse Lsh/Ity/Bcg locus regulates natural resistance to intracellular pathogens, and the Nramp1 gene was isolated as its candidate. Nramp is part of a small family of at least two genes, Nramp1 and Nramp2. In the present study, a full-length cDNA for carp NRAMP has been isolated and characterized. Nucleotide and predicted amino acid sequence analysis indicate that the carp NRAMP encodes a 548 amino acid membrane protein with 12 putative transmembrane domains, two N-linked glycosylation sites, and an evolutionarily conserved consensus transport motif. The peptide sequence identity among carp and human NRAMP2 is 78%, and 65% with human NRAMP1. Reverse transcription-polymerase chain reaction revealed that carp NRAMP is ubiquitously expressed. Phylogenetic analysis, using neigbor-joining, showed that the carp NRAMP protein clustered together with mammalian NRAMP2 proteins.

Key words NRAMP Fish Carp Evolution Expression 

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Jeroen P. J. Saeij
    • 1
  • G. F. Wiegertjes
    • 1
  • René J. M. Stet
    • 1
  1. 1.Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen Agricultural University, P.O. Box 338, 6700 AH Wageningen, The Netherlands e-mail: Geert.Wiegertjes@celb.wau.nl, Tel.: +31-74-82732, Fax: +31-74-83955NL

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