Immunogenetics

, Volume 49, Issue 5, pp 438–445

Organization of the genes encoding the human proteasome activators PA28α and β

  • Derek McCusker
  • Michael Wilson
  • J. Trowsdale
ORIGINAL PAPER

DOI: 10.1007/s002510050517

Cite this article as:
McCusker, D., Wilson, M. & Trowsdale, J. Immunogenetics (1999) 49: 438. doi:10.1007/s002510050517

Abstract

 Two proteasome activators PA28α and β, which have been implicated in antigen processing for loading class I MHC molecules, are synthesized in response to Ifn-γ. The human genes encoding these activators (PSME1 and PSME2, respectively) were analyzed by sequencing. Each gene comprised 11 exons, consistent with gene duplication during vertebrate evolution. The intron/exon organization of both genes was highly conserved, the major difference being the absence of the exon encoding the lysine and glutamic acid-rich 'KEKE' motif in PA28β. Two other genes of relevance to the immune system were located close to those for PA28 at 14q11.2 including ISGF3G, a protein involved in transcription after IFNα signalling. These sequences were also characterized.

Key words PA28ProteasomeGene structureEvolutionHuman

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Derek McCusker
    • 1
  • Michael Wilson
    • 1
  • J. Trowsdale
    • 1
  1. 1.Division of Immunology, Department of Pathology, Cambridge University, Tennis Court Road, Cambridge, CB2 1QP, UK, E-mail: jt233@mole.bio.cam.ac.uk, Tel.: +44-1223-333248, Fax: +44-1223-333875GB