Immunogenetics

, Volume 49, Issue 2, pp 134–141

The production of two Th2 cytokines, interleukin-4 and interleukin-10, is controlled independently by locus Cypr1 and by loci Cypr2 and Cypr3, respectively

  • Marcela Kosařová
  • Helena Havelková
  • Magdaléna Krulová
  • Peter Demant
  • M. Lipoldová
ORIGINAL PAPER

DOI: 10.1007/s002510050472

Cite this article as:
Kosařová, M., Havelková, H., Krulová, M. et al. Immunogenetics (1999) 49: 134. doi:10.1007/s002510050472

Abstract

 The strains BALB/cHeA (BALB/c) and STS/A (STS) differ in production of IL-4 and IL-10, two Th2 cytokines, after stimulation of spleen cells with Concanavalin A, STS being a low and BALB/c a high producer. We analyzed the genetic basis of this strain difference using the recombinant congenic (RC) strains of the BALB/c-c-STS/Dem (CcS/Dem) series. This series comprises 20 homozygous strains. Each CcS/Dem strain contains a different, random set of approximately 12.5% genes of the "donor" strain STS and approximately 87.5% of the "background" strain BALB/c. We selected for further analysis the RC strain production intermediate between BALB/c and STS. In (CcS-20×BALB/c)F2 hybrids we found that different loci control expression of IL-4 and IL-10. Cypr1 (cytokine production 1) on chromosome 16 near D16Mit15 controls IL-4 production, whereas the production of IL-10 is influenced by loci Cypr2 near D1Mit14 and D1Mit227 on chromosome 1 and Cypr3 marked by D5Mit20 on chromosome 5. In addition, the relationship between the level of these two cytokines depends on the genotype of the F2 hybrids at a locus cora1 (correlation 1) on chromosome 5. This differential genetic regulation may be relevant for the understanding of biological effects of T-helper cells in mice of different genotypes.

Key words Interleukin-4Interleukin-10ProductionGeneRegulation

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Marcela Kosařová
    • 1
  • Helena Havelková
    • 1
  • Magdaléna Krulová
    • 1
  • Peter Demant
    • 2
  • M. Lipoldová
    • 1
  1. 1.Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Fleming. nám. 2, 16637 Praha 6, Czech Republic
  2. 2.Division of Molecular Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The NetherlandsNL