Immunogenetics

, Volume 48, Issue 3, pp 163–173

The genomic organization of NKG2C, E, F, and D receptor genes in the human natural killer gene complex

  • Jens Glienke
  • Yuri Sobanov
  • Christine Brostjan
  • Christina Steffens
  • Catherine Nguyen
  • Hans Lehrach
  • E. Hofer
  • F. Francis
ORIGINAL PAPER

DOI: 10.1007/s002510050420

Cite this article as:
Glienke, J., Sobanov, Y., Brostjan, C. et al. Immunogenetics (1998) 48: 163. doi:10.1007/s002510050420

Abstract

 Interactions of natural killer cell receptors with their cognate ligands play a major role in regulating NK cell function. The NKG2 gene family encodes several highly similar proteins, which are known to form heterodimers with the CD94 receptor. These dimers play a role in the inhibition as well as the activation of NK cells. We have analyzed the gene structures of the NKG2C, D, E, and F genes, and determined their genomic organization. Restriction mapping and sequencing revealed the four genes to be closely linked to one another, and of the same transcriptional orientation. An exon duplication within the NKG2C and E genes was identified, although the duplicated version of this exon has not yet been found in mRNA sequences. The NKG2C, E, and F genes, despite being highly similar, are variable at their 3' ends. We show that NKG2C consists of six exons, whereas NKG2E has seven, and the splice acceptor site for the seventh exon occurs in an Alu repeat. NKG2F consists of only four exons and part of exon IV is in some cases spliced to the 5' end of the NKG2D transcript. NKG2D has only a low similarity to the other NKG2 genes.

Key words Natural killer cellsNKG2NK complexGenomic organizationChromosome 12

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Jens Glienke
    • 1
  • Yuri Sobanov
    • 2
  • Christine Brostjan
    • 2
  • Christina Steffens
    • 1
  • Catherine Nguyen
    • 3
  • Hans Lehrach
    • 1
  • E. Hofer
    • 2
  • F. Francis
    • 1
  1. 1.Max Planck Institute for Molecular Genetics, Ihnestrasse 73, D-14195 Berlin, GermanyDE
  2. 2.Department of Vascular Biology and Thrombosis Research, University of Vienna, Brunnerstrasse 59, A-1235 Vienna, AustriaAT
  3. 3.Centre d'immunologie INSERM/CNRS de Marseille-Luminy, F-13288 Marseille Cedex 9, FranceFR