Immunogenetics

, Volume 47, Issue 4, pp 305–309

Structure of the human CD94 C-type lectin gene

  • Antonio Rodríguez
  • Marta Carretero
  • Jens Glienke
  • Teresa Bellón
  • A. Ramírez
  • Hans Lehrach
  • Fiona Francis
  • M. López-Botet
ORIGINAL PAPER

DOI: 10.1007/s002510050362

Cite this article as:
Rodríguez, A., Carretero, M., Glienke, J. et al. Immunogenetics (1998) 47: 305. doi:10.1007/s002510050362

Abstract

 The genomic structure of the human CD94 gene was obtained by analyzing genomic clones obtained from two different libraries. The CD94 gene contains six exons separated by five introns. The carbohydrate-recognition domain (CRD) is encoded by three exons, and the conservation of intron positions within the CRD indicated that CD94 is closely related to group V of C-type lectins. Primer extension and S1 nuclease protection assays showed that initiation of transcription in the CD94 gene is heterogeneous, but restricted to a 60 base pair region around the major initiation site enclosed within a putative initiator element (TTA+1TTCA). The study of the promoter region of CD94 may help to understand the selective expression of this C-type lectin glycoprotein on NK cells and subsets of cytotoxic T cells.

Key words CD94GeneNK cellC-type lectinChromosome 12

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Antonio Rodríguez
    • 1
  • Marta Carretero
    • 1
  • Jens Glienke
    • 2
  • Teresa Bellón
    • 1
  • A. Ramírez
    • 1
  • Hans Lehrach
    • 2
  • Fiona Francis
    • 2
  • M. López-Botet
    • 1
  1. 1.Hospital de la Princesa, Universidad Autónoma de Madrid, Servicio de Inmunología, Diego de León, 62, E-28006 Madrid, SpainES
  2. 2.Max-Planck-Institut für Molekulare Genetik, Berlin (Dahlem), GermanyDE